...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Translational research: the journal of laboratory and clinical medicine >Vesicle-associated microRNAs are released from blood cells on incubation of blood samples
【24h】

Vesicle-associated microRNAs are released from blood cells on incubation of blood samples

机译：孵育血样后，与囊泡相关的microRNA从血细胞中释放出来

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

MicroRNAs (miRNAs) circulating extracellularly in the blood are currently intensively studied as novel disease markers. However, the preanalytical factors influencing the levels of the extracellular miRNAs are still incompletely explored. In particular, it is unknown, whether the incubation of blood samples as occurring in clinical routine can lead to a release of miRNAs from blood cells and thus alter the extracellular miRNA levels before the preparation of serum or plasma from the blood cells. Using a set of marker miRNAs and quantitative RT-PCR, we found that the levels of extracellular miRNA-1, miRNA-16, and miRNA-21 were increased in EDTA and serum collection tubes incubated for 1-3 hours at room temperature and declined thereafter; the levels of the liver-specific miRNA-122 declined monophasically. These events occurred in the absence of significant hemolysis. When the blood was supplemented with Ribonuclease A inhibitor, the levels of miRNA-1, miRNA-16, and miRNA-21 increased substantially during the initial 3 hours of incubation and those of miRNA-122 remained unchanged, indicating that the release of blood cell-derived miRNAs occurred during the initial 3 hours of incubation of the blood tubes, but not at later time points. Separation of 5-hour preincubated blood into vesicle and nonvesicle fractions revealed a selective increase in the portion of vesicle-associated miRNAs. Together, these data indicate that the release of vesicle-associated miRNAs from blood cells can occur in blood samples within the time elapsing in normal clinical practice until their processing without significant hemolysis. This becomes particularly visible on the inhibition of miRNA degradation by Ribonuclease A inhibitor.
机译：目前正在深入研究血液中在细胞外循环的MicroRNA（miRNA）作为新型疾病标记。但是，影响细胞外miRNA水平的分析前因素仍未完全探讨。特别地，尚不清楚在临床常规中进行的血液样品的孵育是否会导致血细胞释放miRNA，从而改变血细胞制备血清或血浆之前的细胞外miRNA水平。使用一组标记物miRNA和定量RT-PCR，我们发现EDTA中的细胞外miRNA-1，miRNA-16和miRNA-21的水平增加，血清收集管在室温下孵育1-3小时，然后下降之后;肝脏特异性miRNA-122的水平单相下降。这些事件发生在没有明显溶血的情况下。当血液中添加核糖核酸酶A抑制剂后，在孵育的最初3小时内，miRNA-1，miRNA-16和miRNA-21的水平显着增加，而miRNA-122的水平则保持不变，表明血细胞释放衍生的miRNA在培养的最初3小时内发生，但在以后的时间点没有发生。将5小时的预温育血液分离到囊泡和非囊泡馏分中，发现与囊泡相关的miRNA的部分选择性增加。总之，这些数据表明，在正常临床实践中直至其处理而没有明显溶血的时间段内，血液样品中可能发生囊泡相关miRNA的释放。这在核糖核酸酶A抑制剂抑制miRNA降解中特别明显。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号