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首页> 外文期刊>Transfusion medicine >Quantitative study of starving platelets in a minimal medium: maintenance by acetate or plasma but not by glucose
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Quantitative study of starving platelets in a minimal medium: maintenance by acetate or plasma but not by glucose

机译：在基本培养基中饥饿的血小板的定量研究：通过乙酸盐或血浆维持血糖，而不通过葡萄糖维持

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summary. The requirement of donor platelets for fuels, plasma and calcium were studied using platelets washed, filtered to remove leucocytes and resuspended in a new gJucose-free minimal platelet storage medium with low citrate (3 mmol/1), low buffer capacity and no calcium. This is the first study of platelets stored without plasma, glucose or calcium and it was shown that platelets continued to aggregate with collagen plus adrenaline for 48 h and showed only a 50% fall in 'swirl index', an objective morphology score, after 3 days, showing that by these criteria human platelets do not require glucose. Sodium acetate extended the storage time by between 2 and 4 days, depending on the indexparameter. This is the first evidence showing that failure of platelets in these conditions is at least partly due to exhaustion of fuel, and the first evidence that acetate prolongs in vitro survival. As little as 10% low-glucose plasma extended the storage time, but it was no better than acetate. New observations using this system included a very rapid fall in pH during resuspension of the washed platelet pellet, a rising pH in the absence of added fuel and an increased pH with added acetate.
机译：概要。研究了供体血小板对燃料，血浆和钙的需求，方法是洗涤血小板，过滤以除去白细胞，然后将其重悬浮于新型的无柠檬酸的最小血小板存储培养基中，该培养基具有低柠檬酸盐（3 mmol / 1），低缓冲容量和无钙。这是对没有血浆，葡萄糖或钙的血小板进行的首次研究，结果显示血小板在48小时内继续与胶原蛋白和肾上腺素聚集，并且显示“涡旋指数”（客观形态评分）仅下降了50％，经过3次天，表明通过这些标准，人体血小板不需要葡萄糖。乙酸钠将存储时间延长了2至4天，具体取决于折射率参数。这是第一个证据表明在这些情况下血小板衰竭至少部分是由于燃料耗尽所致，也是第一个证据表明乙酸盐可以延长体外存活时间。低至10％的低葡萄糖血浆可延长储存时间，但并不比醋酸盐好。使用该系统的新观察结果包括在重新悬浮洗涤后的血小板沉淀过程中pH值迅速下降，在不添加燃料的情况下pH升高以及在添加乙酸盐的情况下pH升高。

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