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首页> 外文期刊>Trends in Plant Science >Imaging protein-protein interactions in plant cells by bimolecular fluorescence complementation assay
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Imaging protein-protein interactions in plant cells by bimolecular fluorescence complementation assay

机译：通过双分子荧光互补测定法成像植物细胞中的蛋白质-蛋白质相互作用

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摘要

The bimolecular fluorescence complementation (BiFC) assay is based on the reconstruction of a fluorescent signal upon the interaction of two protein partners fused to two non-fluorescent fragments of an otherwise fluorescent protein. Interacting partners are typically tagged to fragments of the yellow fluorescent protein, but the use of other fluorescent proteins has been reported. By combining fragments of different types of fluorescent proteins, it is possible not only to detect pairwise protein-protein interaction but also to study the formation of multiprotein complexes in living cells. As we discuss here, a multicolor BiFC set of vectors has been recently deployed for visualizing the simultaneous formation of alternative protein kinase and calcium sensor complexes in living plant cells. This proof-of-concept report and the vectors that have been developed are an important addition to the sets of tools that are useful for analysing multiprotein complexes in plant cells.
机译：双分子荧光互补（BiFC）测定法是基于两个蛋白质伴侣融合到原本荧光蛋白的两个非荧光片段上的相互作用而重建的荧光信号。通常将相互作用的伴侣标记到黄色荧光蛋白的片段上，但已有报道使用其他荧光蛋白。通过组合不同类型的荧光蛋白的片段，不仅可以检测成对的蛋白-蛋白相互作用，而且可以研究活细胞中多蛋白复合物的形成。正如我们在此处讨论的那样，最近已部署了多色BiFC载体集，用于可视化在活植物细胞中同时形成的替代蛋白激酶和钙传感器复合物。该概念验证报告和已开发的载体是对可用于分析植物细胞中多蛋白复合物的一系列工具的重要补充。

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