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Essential nucleoid proteins in early chloroplast development.

机译:叶绿体早期发育中必需的类核蛋白。

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The plastid transcription machinery can be biochemically purified at different organisational levels as soluble RNA polymerase, transcriptionally active chromosome, or nucleoid. Recent proteomic studies have uncovered several novel proteins in these structures and functional genomic studies have indicated that a lack of many of these proteins results in chlorotic phenotypes of varying degree. The most severe cases exhibit complete albino phenotypes, which led to the conclusion that the proteins that were lacking had important regulatory roles in plastid gene expression and chloroplast development. In this opinion article, we propose an alternative model in which the structural establishment of a transcriptional subdomain within the nucleoid represents an early developmental bottleneck that leads to abortion of proper chloroplast biogenesis if disturbed.
机译:质体转录机制可以在不同的组织水平上进行生化纯化,例如可溶性RNA聚合酶,转录活性染色体或核苷。近期的蛋白质组学研究发现了这些结构中的几种新型蛋白质,功能基因组学研究表明,缺乏许多此类蛋白质会导致不同程度的褪绿型表型。最严重的病例表现出完全的白化病表型,这得出的结论是,缺乏的蛋白质在质体基因表达和叶绿体发育中具有重要的调节作用。在这篇观点文章中,我们提出了一种替代模型,其中核苷酸内转录亚结构域的结构建立代表了一个早期的发展瓶颈,如果受到干扰,该瓶颈会导致适当的叶绿体生物发生中止。

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