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首页> 外文期刊>Tropical Animal Health and Production >Effect of addition of coconut water (Cocos nucifera) to the freezing media on post-thaw viability of boar sperm.
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Effect of addition of coconut water (Cocos nucifera) to the freezing media on post-thaw viability of boar sperm.

机译：在冷冻培养基中添加椰子水（椰子）对公猪精子解冻后活力的影响。

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The aims of this experiment were to evaluate the addition of coconut water in natura to the freezing media, compare the effect of deionized water vs filtered water of coconut over the post-thaw seminal characteristics, and evaluate the effect of the deionized water and in natura coconut water on the seminal characteristics of boar sperm at different post-thaw times. Thirty-four ejaculates were used divided in three aliquots which received one of the following treatments (T): T1, LEY (bidistilled water, lactose, and egg yolk) and LEYGO (LEY+glycerol and Orvus ET paste); T2, LEYA (coconut deionized water, lactose, and egg yolk)-LEYGOA; and T3, LEYB (in natura coconut water, lactose, and egg yolk)-LEYGOB. Samples of boar semen were frozen according to the Westendorf method, thawed at 38 degrees C, and evaluated at three incubation times (0, 30, and 60 min). Seminal characteristics assessed were motility (Mot), acrosomal integrity (AInt), membrane integrity (MInt), and mitochondrial activity (MAct). T1 showed a higher percentage of viable sperm than T3 (Mot 36.5 vs 5.4%, AInt 61.8 vs 41.2%, MInt 50.4 vs 41.3%, and MAct 56.9 vs 50.5%). T2 kept a higher percentage of viable sperm at all incubation times. In natura coconut water showed a detrimental effect over the viability of the frozen-thawed boar semen. Deionized coconut water improved the boar semen viability post-thaw, outperforming results of in natura coconut water.
机译：本实验的目的是评估自然介质中的椰子水在冷冻介质中的添加量，比较去离子水与过滤后的椰子水对融化后精液特性的影响，并评估去离子水和自然水中的椰子汁的作用。椰子水对公猪精子在不同解冻时间后精子特性的影响。使用三十四种射精，分成三个等分试样，分别接受以下一种处理（T）：T1，LEY（去水，乳糖和蛋黄）和LEYGO（LEY +甘油和Orvus ET糊剂）; T2，LEY A （椰子去离子水，乳糖和蛋黄）-LEYGO A ;和T3，LEYB（在自然椰子水，乳糖和蛋黄中）-LEYGO B 。根据Westendorf方法将公猪精液样品冷冻，在38摄氏度下解冻，并在三个孵育时间（0、30和60分钟）进行评估。评估的主要特征是运动性（Mot），顶体完整性（AInt），膜完整性（MInt）和线粒体活性（MAct）。 T1的活精子百分比高于T3（Mot 36.5 vs 5.4％，AInt 61.8 vs 41.2％，MInt 50.4 vs 41.3％，MAct 56.9 vs 50.5％）。在所有孵育时间中，T2保留较高百分比的活精子。在natura中，椰子水对冷冻解冻的公猪精液的活力显示出有害作用。去离子椰子水可改善解冻后公猪精液的活力，优于天然椰子水的结果。

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