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首页> 外文期刊>Tropical Animal Health and Production >Diagnosis of classical swine fever virus in a limited resource setting: the influence of pig breed on methodology and sample selection.
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Diagnosis of classical swine fever virus in a limited resource setting: the influence of pig breed on methodology and sample selection.

机译：在资源有限的情况下诊断经典猪瘟病毒：猪的品种对方法和样品选择的影响。

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摘要

This study investigated the influence of pig breed on the diagnosis (serology, antigen detection and gross pathology) of classical swine fever virus (CSFV) in the limited resource setting of Lao People's Democratic Republic (Lao PDR) and aimed to develop a strategy to improve diagnosis of CSF. Moo Laat (ML, an indigenous Lao breed) and Large White x Landrace (LWC) pigs were inoculated with 1 ml of CSFV subcutaneously. Six animals per breed were inoculated with CSF while control animals received saline. Serology and antigen detection using ELISA and gross pathology assessments were performed in each animals. It was shown that ML pigs survived longer than LWC pigs following CSFV infection. Onset of clinical signs and time to death euthanasia were 6 days postinfection and 15-18 days and 4 days postinfection and 8-11 days for ML and LWC pigs, respectively. CSFV seroconversion was not detected except in 1 ML pig. Postmortem gross pathology lesions (ecchymotic or petechial haemorrhages) and antigen levels were significantly higher in ML compared to LWC pigs. The most reliable tissues for antigen detection were spleen (91.7%) and mesenteric lymph nodes (75.0%). Brain tissue was negative for CSFV antigens. This study shows the usefulness of three basic methods for the diagnosis of CSFV infection in a low resource setting.
机译：本研究在老挝人民民主共和国（Lao PDR）的资源有限的情况下，研究了猪品种对经典猪瘟病毒（CSFV）的诊断（血清学，抗原检测和总体病理）的影响，旨在制定改善策略脑脊液的诊断。给Moo Laat（ML，老挝土生土长的品种）和Large White x Landrace（LWC）猪皮下接种1 ml CSFV。每个品种六只动物接种CSF，而对照动物则接受盐水。在每只动物中进行使用ELISA的血清学和抗原检测以及总体病理学评估。结果表明，MLF猪在感染猪瘟病毒后比LWC猪存活更长。 ML和LWC猪的临床体征和安乐死的死亡时间分别为感染后6天，感染后15-18天和4天以及8-11天。除1 ML猪外，未检测到CSFV血清转化。与LWC猪相比，ML的死后总体病理损伤（瘀斑或瘀斑出血）和抗原水平显着更高。用于检测抗原的最可靠的组织是脾脏（91.7％）和肠系膜淋巴结（75.0％）。脑组织CSFV抗原阴性。这项研究表明，在资源匮乏的情况下，三种基本方法可用于诊断CSFV感染。

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