• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Tropical Animal Health and Production >Evaluation of ELISA in the serodiagnosis of bovine farcy
【24h】

Evaluation of ELISA in the serodiagnosis of bovine farcy

机译：酶联免疫吸附测定法在牛粪便血清学诊断中的价值

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Mycobacterium farcinogenes is the causal agent of bovine farcy, a chronic infectious disease of zebu cattle in some parts of tropical Africa. Whole cell homologous antigen of M. farcinogenes was used in the standardization and evaluation of an enzyme linked immunosorbent assay (ELISA) for the detection of circulating antibodies against bovine farcy using sera from confirmed bovine farcy and from bovine farcy-free cattle. The cut-off optical density (OD) value was decided at 1.8 using filter 405nm after one hour of incubation at 37pC. Accordingly, 115 out of 124 (92.7%) serum samples from clinically proven bovine farcy cattle were reported sero-positive. Sera from cattle infected with M. avium and M. paratuberculosis revealed OD value <1.8, indicating the differential diagnostic ability of M. farcinogenes antigen. Our test sensitivity was 92.7% and specificity was 97%, therefore could be routinely employed to support early clinical diagnosis, epidemiological surveys and for screening animals before exportation to farcy-free regions.
机译：分支杆菌分枝杆菌是牛分支杆菌的病原体，牛分支杆菌是热带非洲某些地区的封牛的一种慢性传染病。产乳链球菌的全细胞同源抗原用于酶联免疫吸附测定（ELISA）的标准化和评估，以使用来自确认的牛粪和无牛粪的牛血清检测抗牛粪的循环抗体。在37pC下孵育1小时后，使用405nm滤光片将截止光密度（OD）值确定为1.8。因此，从临床证明的牛驼毛牛的124份血清样品中，有115份（92.7％）据报道是血清阳性的。来自被鸟分枝杆菌和副结核分枝杆菌感染的牛的血清显示OD值<1.8，表明杆状分枝杆菌抗原的鉴别诊断能力。我们的测试敏感性为92.7％，特异性为97％，因此可以常规用于支持早期临床诊断，流行病学调查以及在出口到无绒毛地区之前对动物进行筛查。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号