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首页> 外文期刊>Tropical Animal Health and Production >Pathology and molecular diagnosis of paratuberculosis of camels.
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Pathology and molecular diagnosis of paratuberculosis of camels.

机译：骆驼副结核的病理学和分子诊断。

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Camels are the prime source of meat and milk in many desert regions of the world including Saudi Arabia. Paratuberculosis of camels, locally called Silag, is a serious and invariably fatal disease in the Arabian camel. Six camels were used in this study. Five camels with clinical paratuberculosis were used to study the pathology of the disease and confirm its aetiology. The sixth camel was clinically healthy and used as a control. The camels were examined clinically and bled for haematological and blood chemistry analysis. They were then humanely killed with a high intravenous dose of thiopental sodium (10 mg/kg) for pathological studies as well as obtaining tissues for microbiological and molecular studies. The clinical signs of the disease were emaciation, diarrhoea, alopecia, wry neck and pale mucous membranes. Laboratory diagnosis showed reduced haemoglobin concentration, low haematocrit and high activity of the serum enzyme alanine aminotransferase. Serum creatinine concentration was normal. These results indicated the infected camels were anaemic and the function of their livers was affected. Postmortem examination showed thickened and corrugated intestinal mucosa, enlarged granulomatous mesenteric lymph nodes, miliary and diffuse granulomas in the liver (in four camels), generalized lymph node granulomas (in one camel), splenic granuloma (in one camel) and mediastinal lymph node granuloma (in two camels). Histopathological examination showed diffuse infiltration of macrophages in all organs showing lesions. Ziehl-Neelsen staining of tissue scraping and tissue sections showed masses of acid fast bacilli, except for the spleen. Infection with Mycobacterium avium subsp. paratuberculosis was confirmed by PCR by targeting the IS900 gene.
机译：骆驼是包括沙特阿拉伯在内的世界许多沙漠地区的主要肉类和牛奶来源。骆驼副结核病，在当地被称为锡拉格（Silag），是阿拉伯骆驼中的一种严重且总是致命的疾病。在这项研究中使用了六只骆驼。使用五只骆驼和临床上的副结核病来研究该疾病的病理学并确认其病因。第六只骆驼在临床上健康，可以用作对照。对骆驼进行了临床检查，并进行了血液和血液化学分析。然后用高剂量的硫喷妥钠（10 mg / kg）静脉内杀死他们，进行病理学研究，并获得组织进行微生物学和分子学研究，以人道方式将其杀死。该病的临床体征为消瘦，腹泻，脱发，颈扭和粘膜苍白。实验室诊断显示血红蛋白浓度降低，血细胞比容低和血清丙氨酸转氨酶活性高。血清肌酐浓度正常。这些结果表明被感染的骆驼贫血，并且其肝功能受到影响。验尸检查显示肠粘膜增厚和呈波纹状，肠系膜肉芽肿淋巴结肿大，肝脏粟粒和弥漫性肉芽肿（4头骆驼），全身淋巴结肉芽肿（1头骆驼），脾肉芽肿（1头骆驼）和纵隔淋巴结肉芽肿（两只骆驼）。组织病理学检查显示所有病变均可见巨噬细胞浸润。 Ziehl-Neelsen组织刮取和组织切片染色显示除脾脏外有大量耐酸杆菌。禽分枝杆菌亚种感染。通过靶向IS 900 基因的PCR证实了肺结核。

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