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首页> 外文期刊>Tropical Animal Health and Production >Antimicrobial susceptibility of lactic acid bacteria isolated from human and food-producing animal feces in Khon Kaen Province, Thailand
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Antimicrobial susceptibility of lactic acid bacteria isolated from human and food-producing animal feces in Khon Kaen Province, Thailand

机译：从泰国孔敬省人类和食用动物粪便中分离出的乳酸菌的抗菌敏感性

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The aim of this study was to investigate the susceptibility of 93 Lactobacillus strains to seven antimicrobial agents, i.e., penicillin G, amoxicillin-clavulanic acid, vancomycin, tetracycline, streptomycin, ciprofloxacin, and sulfamethoxazole-trimethoprim, by disk diffusion test. The Lactobacillus strains were isolated from fecal samples taken from 90 healthy, food-producing animals (fattening pigs, free-grazing ducks, and beef cattle) and 30 healthy human subjects (1- to 6-year-olds) in Khon Kaen. The minimum inhibitory concentration (MIC) values of tetracycline and ciprofloxacin against all strains were determined using the E-test. All 93 Lactobacillus isolates were identified at the species level using 16S rRNA gene sequencing. The most common species of Lactobacillus isolated from fattening pigs, free-grazing ducks, beef cattle, and humans were L. reuteri (30 %), L. salivarius (46.7 %), L. acetotolerans (20 %), and L. gasseri (33.3 %), respectively. A total of 83 Lactobacillus strains were resistant to the examined antibiotics. Some strains were resistant to two to six types of antibiotics. More than 50 % of Lactobacillus species were intrinsically resistant to vancomycin, streptomycin, ciprofloxacin, and sulfamethoxazole-trimethoprim. The prevalence of acquired resistance to tetracycline was observed for Lactobacillus isolates from fattening pigs, humans, free-grazing ducks, and beef cattle at 92.3, 85.7, 77.8, and 68.4 %, respectively. These results demonstrate the impact of antibiotic use in human and veterinary medicine on antibiotic treatment efficacy and may support the spread of transferable antibiotic resistant genes to other bacteria via the food chain.
机译：这项研究的目的是通过纸片扩散试验研究93株乳酸菌对7种抗微生物剂的敏感性，即青霉素G，阿莫西林-克拉维酸，万古霉素，四环素，链霉素，环丙沙星和磺胺甲恶唑-三甲氧嘧啶。从乳酸菌菌株中分离出粪便样品，这些粪便样品取自孔敬的90例健康，可食用的动物（肥猪，放牧鸭和肉牛）和30例健康的人类受试者（1至6岁）。使用E检验确定四环素和环丙沙星对所有菌株的最小抑菌浓度（MIC）值。使用16S rRNA基因测序在物种水平上鉴定了所有93株乳酸杆菌。从育肥猪，放牧鸭，肉牛和人中分离出的最常见的乳杆菌是罗伊氏乳杆菌（30％），唾液乳杆菌（46.7％），醋酸小乳杆菌（20％）和加氏乳杆菌（33.3％）。总共83株乳酸菌对所检查的抗生素具有抗性。一些菌株对两种到六种抗生素具有抗性。超过50％的乳杆菌对万古霉素，链霉素，环丙沙星和磺胺甲恶唑-甲氧苄啶具有内在抗性。从育肥猪，人，放牧鸭和肉牛中分离出的乳杆菌分离株观察到获得的对四环素抗性的流行率分别为92.3％，85.7、77.8和68.4％。这些结果证明了在人类和兽医学中使用抗生素对抗生素治疗功效的影响，并且可能支持通过食物链将可转移的抗生素抗性基因传播到其他细菌。

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