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首页> 外文期刊>Tropical Animal Health and Production >Real-time PCR carried out on DNA extracted from serum or blood sample is not a good method for surveillance of bovine brucellosis
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Real-time PCR carried out on DNA extracted from serum or blood sample is not a good method for surveillance of bovine brucellosis

机译：对从血清或血液样本中提取的DNA进行实时PCR并不是监测牛布鲁氏菌病的好方法

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摘要

Bovine brucellosis is endemic in many parts of the world including India. The disease diagnosis and surveillance are usually carried out by serological tests, which however have drawbacks. This study was undertaken to evaluate the potential of real-time PCR (RT-PCR) targeting bcsp31 gene for surveillance of bovine brucellosis. A total of 461 samples, which included 408 stored serum and 53 prospective blood samples, were used. It was found that 33 (7.15 %) samples were positive by RT-PCR, whereas 149 (32.32 %) and 132 (28.63 %) were positive by Rose Bengal plate test (RBPT) or standard agglutination test (STAT), respectively. The results of this study suggest that RT-PCR targeting bcsp31 gene carried out on DNA extracted from serum or blood may not be a suitable method for surveillance of brucellosis in bovines.
机译：牛布鲁氏菌病在世界许多地方都流行，包括印度。疾病的诊断和监视通常通过血清学检查来进行，但是有缺点。进行这项研究来评估靶向bcsp31基因的实时PCR（RT-PCR）监测牛布鲁氏菌病的潜力。总共使用了461个样本，其中包括408个存储的血清和53个预期的血液样本。通过RT-PCR检测到33份（7.15％）样品为阳性，而通过Rose Bengal平板试验（RBPT）或标准凝集试验（STAT）分别为149（32.32％）和132（28.63％）为阳性。这项研究的结果表明，针对从血清或血液中提取的DNA进行bcsp31基因靶向的RT-PCR可能不是监测牛布鲁氏菌病的合适方法。

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