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首页> 外文期刊>Veterinary Microbiology >Short communication: Rapid diagnosis and quantification of Francisella tularensis in organs of naturally infected common squirrel monkeys (Saimiri sciureus)
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Short communication: Rapid diagnosis and quantification of Francisella tularensis in organs of naturally infected common squirrel monkeys (Saimiri sciureus)

机译：简短交流：在自然感染的普通松鼠猴（Saimiri sciureus）的器官中快速诊断和量化土拉弗朗西斯菌

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摘要

Francisella tularensis, a small Gram-negative facultative intracellular bacterium, is the causative agent of tularaemia, a severe zoonotic disease transmitted to humans mostly by vectors such as ticks, flies and mosquitoes. The disease is endemic in many parts of the northern hemisphere. Among animals, the most affected species belong to rodents and lagomorphs, in particular hares. However, in the recent years, many cases of tularaemia among small monkeys in zoos were reported. We have developed a real-time PCR that allows to quantify F. tularensis in tissue samples. Using this method, we identified the spleen and the kidney as the most heavily infected organ containing up to 400 F. tularensis bacteria per simian host cell in two common squirrel monkeys (Saimiri sciureus) from a zoo that died of tularaemia. In other organs such as the brain, F. tularensis was detected at much lower titres. The strain that caused the infection was identified as F. tularensis subsp. holarctica biovar I, which is susceptible to erythromycin. The high number of F. tularensis present in soft organs such as spleen, liver and kidney represents a high risk for persons handling such carcasses and explains the transmission of the disease to a pathologist during post-mortem analysis. Herein, we show that real-time PCR allows a reliable and rapid diagnosis of F. tularensis directly from tissue samples of infected animals, which is crucial in order to attempt accurate prophylactic measures, especially in cases where humans or other animals have been exposed to this highly contagious pathogen.
机译：图兰弗朗西斯菌（Francisella tularensis）是一种革兰氏阴性兼性细胞内小型细菌，是图拉菌血症的病原体，图拉菌血症是一种严重的人畜共患病，主要通过tick，蝇和蚊子等媒介传播给人。该病在北半球许多地方流行。在动物中，受影响最大的物种是啮齿动物和兔形目动物，特别是野兔。然而，近年来，在动物园的小猴子中报告了许多图拉血病病例。我们已经开发了一种实时定量PCR，可以定量组织样品中的T. tularensis。使用这种方法，我们确定了脾脏和肾脏是感染最严重的器官，在一家死于图雷拉虫病的动物园里的两只普通松鼠猴（Saimiri sciureus）中，每个猿猴宿主细胞中含有高达400 F. tularensis细菌。在其他器官（如大脑）中，检测到土拉弗朗西斯滴度要低得多。引起感染的菌株被鉴定为土拉弗朗西斯菌。 holarctica biovar I，易患红霉素。存在于诸如脾脏，肝脏和肾脏等软器官中的土拉弗朗西斯菌数量很高，这对处理此类屠体的人构成了高风险，并在验尸分析期间将疾病传播给了病理学家。本文中，我们显示实时PCR可以直接从受感染动物的组织样本中可靠快速诊断出T. tularensis，这对于尝试采取准确的预防措施至关重要，尤其是在人类或其他动物已经暴露于这种情况下。这种高度传染性的病原体。

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