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One simple and rapid method for evaluating acrosome reaction of giant panda and other mammalian sperm

机译：一种简单快速的评估大熊猫和其他哺乳动物精子顶体反应的方法

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摘要

Similar to other mammals, the giant panda sperms have to undergo capacitation and acrosome reaction (AR) before being able to fertilize the eggs. The sperm ability undergoing acrosome reaction can be regarded as one of the semen quality indexes, but the acrosomes of the giant panda and most of the other mammalian sperms are too small for the acrosomal status to be detected by a light microscope. Although this can be done by transmission electron microscope (TEM), the long time required, the expensivecost and the incapability of distinguishing the physiological and degenerative AR preclude its routine use. The researchers have long been trying to establish simple and rapid methods for detecting acrosome reactions of the human and mammalian sperms atlight microscopic level in recent years. Several techniques, including the chlortetracycline (CTC) fluorescence assay used in mouse and human, the triple stain technique used in human and other mammals, FITC-labeled PSA (FITC-PSA) staining technique used in human and indirect immunofluorescence using monoclonal antibodies, have been proposed to differentiate acrosome-intact from acrosome-reacted sperms by a light microscope. In the present report, a method for detecting the AR of the giant panda and mouse sperms using FITC-ConA or FITC-LCA is described and discussed.
机译：与其他哺乳动物相似，大熊猫精子必须先进行能化和顶体反应（AR），然后才能使卵受精。经历顶体反应的精子能力可以看作是精液质量指标之一，但是大熊猫的顶体和大多数其他哺乳动物的精子太小，无法通过光学显微镜检测到顶体状态。尽管这可以通过透射电子显微镜（TEM）来完成，但所需时间长，成本高以及无法区分生理性和退化性AR，因此无法常规使用。近年来，研究人员一直在尝试建立简单，快速的方法，以在光学显微镜下检测人类和哺乳动物精子的顶体反应。几种技术，包括用于小鼠和人类的金霉素（CTC）荧光测定，用于人类和其他哺乳动物的三重染色技术，用于人类的FITC标记PSA（FITC-PSA）染色技术以及使用单克隆抗体的间接免疫荧光技术，都有有人提出用光学显微镜将完整的顶体与顶体反应的精子区分开。在本报告中，描述并讨论了使用FITC-ConA或FITC-LCA检测大熊猫和小鼠精子的AR的方法。

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