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首页> 外文期刊>Chinese science bulletin >Deletion analysis of oligomycin PKS genes (olmA) in Streptomyces avermitilis
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Deletion analysis of oligomycin PKS genes (olmA) in Streptomyces avermitilis

机译：阿维链霉菌中寡霉素PKS基因（olmA）的缺失分析

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摘要

Gene deletion vector pXL05(pKC1139::△olmA1 +△olmA4) was used to disrupt oligomycin PKS encoding genes(olmA) in Streptomyces avermitilis CZ8-73, the producer of anthelmintic avermectins B and the cell growth inhibitor oligomycin. olmA gene cluster inthe chromosome was displaced by deletion allelc on the plasmid via double crossover. Four of disruptants were confirmed by Southern blotting. Shaking flask experiments and HPLC analyses showed that the four mutants no longer produced the toxic oligomycin, hut only made four components of avermectins B, which were avermectin B1a, B1b, B2a, B2b. The yields of avermectins B in these mutants were separately equal to those in CZ8-73. This revealed that olmA genes deletion did not affect the biosynthesis ofavermectins. The deletion mutants were proved to be genetically stable, and thus might be promising strains in industrial production of avermectins B.
机译：基因缺失载体pXL05（pKC1139 ::△olmA1 +△olmA4）被用来破坏除虫链霉菌A和细胞生长抑制剂寡霉素的阿维链霉菌CZ8-73中编码寡核苷酸的PKS基因（olmA）。染色体上的olmA基因簇通过两次交换被质粒上的缺失等位基因取代。通过Southern印迹证实了四个破坏物。摇瓶实验和HPLC分析表明，这四个突变体不再产生有毒的寡霉素，而仅产生了阿维菌素B的四个成分，即阿维菌素B1a，B1b，B2a，B2b。这些突变体中阿维菌素B的产量分别等于CZ8-73。这表明olmA基因缺失不影响阿维菌素的生物合成。事实证明，该缺失突变体具有遗传稳定性，因此在阿维菌素B的工业生产中可能是有希望的菌株。

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