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首页> 外文期刊>Chinese science bulletin >Construction and characteristics of a transformed lepidopteran cell clone expressing baculovirus p35
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Construction and characteristics of a transformed lepidopteran cell clone expressing baculovirus p35

机译：杆状病毒p35转化鳞翅目细胞克隆的构建及特性

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摘要

A transformed cell line was constructed from Mythimna separata cells Ms7311 by lipofection method. TMs7311 cells were generated using a double selection technique involving a selection in the antibiotic Zeocin, followed by a second round of selection by exhibiting cell characterization. A cell clone expressing p35 was obtained with high level of AcMNPV and recombinant proteins. Compared with wild type Ms7311 cells, the cell clone showed increased resistance to Actinamycin D-induced apoptosis and a profound resistance to nutrient development (PBS). When the cell clone was infected with recombimant baculoviruses expressing secreted alkaline phosphatase (SEAP) and P-galactosidase, expression of the recombinant proteins from TMs7311 cells exceeded that from parental Ms7311 cells. Production of budded virus and occlusion body was significantly higher than that from parental cells Ms7311.
机译：通过脂质转染法从分离的Mythimna separata细胞Ms7311构建转化的细胞系。 TMs7311细胞是使用双重选择技术生成的，该技术涉及在抗生素Zeocin中进行选择，然后通过展示细胞特征进行第二轮选择。获得具有高水平的AcMNPV和重组蛋白的表达p35的细胞克隆。与野生型Ms7311细胞相比，该细胞克隆显示出对放线菌素D诱导的细胞凋亡增加的抵抗力，以及对养分发育（PBS）的深刻抵抗力。当细胞克隆被表达分泌型碱性磷酸酶（SEAP）和P-半乳糖苷酶的重组杆状病毒感染时，TMs7311细胞中重组蛋白的表达超过了亲代Ms7311细胞中的表达。发芽的病毒和闭塞体的产生显着高于亲代细胞Ms7311。

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