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vga mutants of Kluyvermyces lactis show cell integrity defects

机译：乳酸克鲁维酵母的vga突变体显示细胞完整性缺陷

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We studied the cell wall alterations that occur in mutants of Kluyveromyces acts impaired in glycosylation. The mutants belong'to four complementation groups named vgal to vga4 (yanadate glycosylation affected), characterized by sodium orthovanadate resistance and alteration of the glycosylation profile of native invertase. A drastic reduction of the alkalisoluble fraction of the #beta#-D-glucan was observed in vgal, vga2 and vga3 cells, accompanied by an increase in the chitin content of the cell wail. In vga4 cells, both #beta#-D-glucau fractions (alkali-soluble and alkali-insoluble) were reduced to about half of the corresponding wildtype value but the chitin content was normal. A protein related to Fkslp, the catalytic subunit of the major 1,3-#beta#-D-glucan synthase of S. cerevisiae, was detected in K. acts. The amount of this Fkslp-like protein increased 7-10 times in vgal, vga2 and vga3 mutants as compared to wild-type cells; the same strains released significant amounts of #beta#-D-glucan in the culture supernatant. These mutations also resulted in abnormally thick cell walls with conspicuous irregularities in the structure, as revealed by electron microscopy and by an altered resistance to Zymolyase. The observed high responsiveness of cell wall phenotypes to alterations of glycosylation make K. acts an attractive system for studying the interconnections between these processes.
机译：我们研究了克鲁维酵母突变体中糖基化受损的细胞壁改变。该突变体属于四个互补组，称为vgal至vga4（受影响的山梨糖基化），其特征在于原钒酸钠抗性和天然转化酶的糖基化谱改变。在vgal，vga2和vga3细胞中观察到＃beta＃-D-葡聚糖的碱溶性分数急剧降低，同时伴随着细胞壁中几丁质含量的增加。在vga4细胞中，两个＃beta＃-D-glucau馏分（碱溶性和碱溶性）均降低至相应野生型值的一半左右，但几丁质含量正常。在啤酒酵母中检测到与Fkslp有关的蛋白质，Fkslp是酿酒酵母主要1，3-＃β＃-D-葡聚糖合酶的催化亚基。与野生型细胞相比，在vgal，vga2和vga3突变体中，这种Fkslp样蛋白的量增加了7-10倍;同样的菌株在培养上清液中释放出大量的＃beta＃-D-葡聚糖。这些突变还导致异常厚的细胞壁，并在结构上具有明显的不规则性，这通过电子显微镜检查和通过改变对Zymolyase的抗性所揭示。观察到的细胞壁表型对糖基化改变的高响应性使K.起着吸引人的作用，用于研究这些过程之间的相互联系。

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