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DNA purification and gene typing: Based on multifunctional nanobeads

机译:DNA纯化和基因分型:基于多功能纳米珠

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摘要

In this report, a universal protocol for extracting genomic DNA from whole blood, saliva, and bacterial culture by using magnetic nanobeads as solid-phase absorbents was presented. The enrichment of target cells and adsorption of DNA have been functionally integrated onto the surfaces of the carboxyl-modified magnetic nano-beads, and the DNA segments bound on the surface of the beads can be directly used as PCR templates to amplify a target gene. The PCR products were applied to an oligonucleotide array to perform gene typing. The protocol proves to be simple, rapid, biologically and chemically nonhazardous, and promising for the microfabrication of DNA preparation chip.
机译:在本报告中,提出了一种通用协议,该协议通过使用磁性纳米珠作为固相吸收剂从全血,唾液和细菌培养物中提取基因组DNA。靶细胞的富集和DNA的吸附已在功能上整合到羧基修饰的磁性纳米珠的表面上,结合在珠表面上的DNA片段可直接用作PCR模板以扩增靶基因。将PCR产物应用于寡核苷酸阵列以进行基因分型。该协议被证明是简单,快速,对生物和化学无害的,并且有望用于DNA制备芯片的微细加工。

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