Isolation, purification and characterization of a new organphosphorus hydrolase OPHC2

WU Ningfeng; DENG Minjie; SHI Xiuyun; LIANG Guoyi; YAO Bin; FAN Yunliu

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Isolation, purification and characterization of a new organphosphorus hydrolase OPHC2

机译：新型有机磷水解酶OPHC2的分离，纯化和表征

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A bacterium with the capability of degrading organphosphorus, identified as Pseudomonas pseudoaicali-genes, is isolated from OP-treated soil. The organphosphorus hydrolase OPHC2 from this bacterium has been purified and characterized. OPHC2 has optimum activity for the reaction at 65 deg C and pH 9.0 with methyl parathion as a substrate, it also shows good thermal and pEI stability. Most metal ions and chemicals have no effect on the activity of OPHC2. The analyses of nucleotide sequence encoding OPHC2 and amino acid sequence of OPHC2 show that there are lower homologies with those of organphosphorus hydrolase reported in GenBank.

机译：从OP处理过的土壤中分离出一种具有降解器官磷能力的细菌，被鉴定为Pseudomonas pseudoaicali基因。来自该细菌的有机磷水解酶OPHC2已被纯化和鉴定。 OPHC2对于以甲基对硫磷为底物在65摄氏度和pH 9.0下的反应具有最佳活性，并且还显示出良好的热稳定性和pEI稳定性。大多数金属离子和化学物质对OPHC2的活性没有影响。对编码OPHC2的核苷酸序列和OPHC2的氨基酸序列的分析表明，与GenBank报道的有机磷水解酶的同源性较低。

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来源
《Chinese science bulletin》 |2004年第3期|共5页
作者
WU Ningfeng; DENG Minjie; SHI Xiuyun; LIANG Guoyi; YAO Bin; FAN Yunliu;
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正文语种 eng
中图分类自然科学现状及发展;
关键词
Pseudomonas pseudoalcaligenes; organphosphorus (OP); organphosphorus hydrolase (OPH); purification; properties;

机译：伪假单胞菌;有机磷（OP）;有机磷水解酶（OPH）;纯化;性质;

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专利

1. Isolation, purification and characterization of a new organphosphorus hydrolase OPHC2 [J] . WU Ningfeng, DENG Minjie, SHI Xiuyun, Chinese science bulletin . 2004,第3期

机译：新型有机磷水解酶OPHC2的分离，纯化和表征
2. Expression of organophosphorus hydrolase OPHC2 in Pichia pastoris: Purification and characterization [J] . Chu XY, Wu NF, Deng MJ, Protein Expression and Purification . 2006,第1期

机译：有机磷水解酶OPHC2在毕赤酵母中的表达：纯化和鉴定
3. Isolation of poly(beta-L-malic acid)-degrading bacteria and purification and characterization of the PMA hydrolase from Comamonas acidovorans strain 7789. [J] . Godde C, Steinbuchel A, Liebergesell M FEMS Microbiology Letters . 1999,第2期

机译：分离聚（β-L-苹果酸）的细菌，并从Comomonas acidovorans菌株7789中纯化和表征PMA水解酶。
4. Isolation, Purification and Characterization of β-amylase from Dioscorea hispida Dennst [C] . Dwita Oktiarni, Lusiana, Febri Yanti Simamora, International Conference on Mathematics and Natural Sciences . 2015

机译：二磷酸稻田腹腔β-淀粉酶的分离，纯化和表征
5. Cloning, expression, purification, and characterization of a glycoside hydrolase family 44 cellulase from Clostridium acetobutylicum in Escherichia coli. [D] . Shilling, Taran Christopher. 2007

机译：从大肠杆菌中的丙酮丁醇梭菌糖苷水解酶家族44纤维素酶的克隆，表达，纯化和表征。
6. Purification and characterization of a novel enzyme alpha-neoagarooligosaccharide hydrolase (alpha-NAOS hydrolase) from a marine bacterium Vibrio sp. strain JT0107. [O] . Y Sugano, H Kodama, I Terada, 1994

机译：从海洋细菌弧菌中纯化和鉴定新型酶α-新葡糖寡糖水解酶（α-NAOS水解酶）。菌株JT0107。
7. Purification and characterization of a novel enzyme, alpha-neoagarooligosaccharide hydrolase (alpha-NAOS hydrolase), from a marine bacterium, Vibrio sp. strain JT0107. [O] . Sugano, Y, Kodama, H, Terada, I, 1994

机译：从海洋细菌弧菌中纯化和鉴定新型酶，α-新葡糖寡糖水解酶（α-NAOS水解酶）。菌株JT0107。
8. Isolation and Purification of a Diisopropyl Phosphorofluoridate Hydrolase from Thermophilic Bacteria [R] . Walls, L. H. 1987

机译：从嗜热细菌中分离和纯化二异丙基磷酰氟水解酶

Isolation, purification and characterization of a new organphosphorus hydrolase OPHC2

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