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首页> 外文期刊>Helgoland marine research >Identification of cephalopod species from the North and Baltic Seas using morphology, COI and 18S rDNA sequences
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Identification of cephalopod species from the North and Baltic Seas using morphology, COI and 18S rDNA sequences

机译：使用形态学，COI和18S rDNA序列鉴定北海和波罗的海的头足类

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摘要

We morphologically analyzed 79 cephalopod specimens from the North and Baltic Seas belonging to 13 separate species. Another 29 specimens showed morphological features of either Alloteuthis mediaor Alloteuthis subulata or were found to be in between. Reliable identification features to distinguish between A. media and A. subulata are currently not available. The analysis of the DNA barcoding region of the COI gene revealed intraspecific distances (uncorrected p) ranging from 0 to 2.13 % (average 0.1 %) and interspecific distances between 3.31 and 22 % (average 15.52 %). All species formed monophyletic clusters in a neighbor-joining analysis and were supported by bootstrap values of a parts per thousand yen99 %. All COI haplotypes belonging to the 29 Alloteuthis specimens were grouped in one cluster. Neither COI nor 18S rDNA sequences helped to distinguish between the different Alloteuthis morphotypes. For species identification purposes, we recommend the use of COI, as it showed higher bootstrap support of species clusters and less amplification and sequencing failure compared to 18S. Our data strongly support the assumption that the genus Alloteuthis is only represented by a single species, at least in the North Sea. It remained unclear whether this species is A. subulata or A. media. All COI sequences including important metadata were uploaded to the Barcode of Life Data Systems and can be used as reference library for the molecular identification of more than 50 % of the cephalopod fauna known from the North and Baltic Seas.
机译：我们对来自北海和波罗的海的79种头足类标本进行了形态学分析，这些标本属于13个单独的物种。另有29个标本显示了变种或变种的形态特征。目前尚不存在用于区分A.培养基和Sub。a。subulata的可靠识别功能。对COI基因的DNA条形码区域的分析显示，种内距离（未校正的p）为0至2.13％（平均0.1％），种间距离为3.31至22％（平均15.52％）。所有物种在邻居加入分析中均形成了单系簇，并受到每千分之一的自举值的支持，即99％。属于29个异地标本的所有COI单倍型都被分组为一个簇。 COI或18S rDNA序列均无助于区分不同的异型变种。出于物种识别的目的，我们建议使用COI，因为与18S相比，COI显示出对物种簇的更高的自举支持，并且扩增和测序失败更少。我们的数据有力地支持了这样的假设，即至少在北海，异地杜鹃属仅由一个物种代表。尚不清楚该物种是次生曲霉还是中生曲霉。包括重要元数据在内的所有COI序列均已上载到生命数据条形码系统，并可作为参考库用于分子鉴定北海和波罗的海已知50％以上的头足类动物。

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