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Effect of warm and cold stratification and ethanol treatment on germination of Corylopsis seeds

机译：冷热分层和乙醇处理对紫花种子萌发的影响

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摘要

Seed germination experiments with Corylopsis coreana, C. sinensis var. calvescens, and Corylopsis gotoana were conducted. Corylopsis coreana seeds were treated at 5, 7.5, 10, 12.5 and 20 degrees C to study the upper temperature limit to break dormancy. Seeds of C. sinensis var. calvescens were treated 1 month with warm stratification (WS) at 10, 15, 20, and 25 degrees C followed by cold stratification (CS) at 5 degrees C for 0, 1, 2, and 3 months to understand the requirements of WS and CS to effectively break dormancy. Germination of Corylopsis seeds at 15-20 degrees C without CS suggests that dormancy is considered shallow and the upper temperature limit to break is 12.5 degrees C. Immersing seeds in water or ethanol induced seed germination which may result from leaching of inhibitors from the seed. Observation of seed coats following treatment with water and ethanol by means of low temperature scanning electron microscopy (LT-SEM) revealed elongated and rectangular/hexagonal shaped cells in C. coreana and irregular jigsaw puzzle-piece shapes in C. gotoana. In conclusion, dormancy in Corylopsis is considered shallow and the optimum CS is suggested to be 7.5 degrees C, while the optimum WS is ranging from 12.5 to 20 degrees C.
机译：Corylopsis coreana，C. sinensis var。的种子发芽实验进行了小牛犊和果蝇。在5、7.5、10、12.5和20摄氏度下处理红皮菊种子以研究打破休眠的上限温度。中华C种子犊牛在10、15、20和25摄氏度下进行了1个月的温热分层（WS），然后在5摄氏度下进行了冷分层（CS）0、1、2和3个月，以了解WS和CS可以有效打破休眠状态。在没有CS的15-20摄氏度下，库拉索种子的萌发表明休眠被认为是浅的，打破的上限温度是12.5摄氏度。将种子浸入水或乙醇中会导致种子萌发，这可能是由于从种子中浸出抑制剂造成的。用水和乙醇处理后，通过低温扫描电子显微镜（LT-SEM）观察种皮，发现在C. coreana中有细长的矩形/六角形细胞，而在C. gotoana中有不规则的拼图形状。综上所述，干燥症的休眠被认为较浅，建议的最佳CS为7.5摄氏度，而最佳WS为12.5至20摄氏度。

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