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首页> 外文期刊>Human and Experimental Toxicology >An investigation of the effect of thiamine pyrophosphate on cisplatin-induced oxidative stress and DNA damage in rat brain tissue compared with thiamine: Thiamine and thiamine pyrophosphate effects on cisplatin neurotoxicity
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An investigation of the effect of thiamine pyrophosphate on cisplatin-induced oxidative stress and DNA damage in rat brain tissue compared with thiamine: Thiamine and thiamine pyrophosphate effects on cisplatin neurotoxicity

机译：与硫胺素相比，硫胺素焦磷酸对顺铂诱导的大鼠脑组织氧化应激和DNA损伤的影响的研究：硫胺素和硫胺素焦磷酸对顺铂神经毒性的影响

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This study investigated the effects of thiamine pyrophosphate (TPP) at dosages of 10 and 20 mg/kg on oxidative stress induced in rat brain tissue with cisplatin and compared this with thiamine. Cisplatin neurotoxicity represents one of the main restrictions on the drug being given in effective doses. Oxidative stress is considered responsible for cisplatin toxicity. Our results showed that cisplatin increased the levels of oxidant parameters such as lipid peroxidation (thio barbituric acid reactive substance (TBARS)) and myeloperoxidase (MPO) in brain tissue and suppressed the effects of antioxidants such as total glutathione (GSH) and superoxide dismutase (SOD). TPP, especially at a dosage of 20 mg/kg, significantly reduced TBARS and MPO levels that increase with cisplatin administration compared with the thiamine group, while TPP significantly increases GSH and SOD levels. In addition, the level of 8-Gua (guanine), a product of DNA damage, was 1.7 ± 0.12 8-hydroxyl guanine (8-OH Gua)/105 Gua in brain tissue in the control group receiving cisplatin, compared with 0.97 ± 0.03 8-OH Gua/105 Gua in the thiamine pyrophosphate (20 mg/kg) group and 1.55 ± 0.11 8-OH Gua/105 Gua in the thiamine (20 mg/kg) group. These results show that thiamine pyrophosphate significantly prevents oxidative damage induced by cisplatin in brain tissue, while the protective effect of thiamine is insignificant.
机译：这项研究调查了硫胺素焦磷酸酯（TPP）在10和20 mg / kg的剂量下对顺铂在大鼠脑组织中诱发的氧化应激的影响，并将其与硫胺素进行了比较。顺铂的神经毒性代表了以有效剂量给予药物的主要限制之一。氧化应激被认为是顺铂毒性的原因。我们的研究结果表明，顺铂可增加脑组织中脂质过氧化（硫代巴比妥酸反应性物质（TBARS））和髓过氧化物酶（MPO）等氧化剂参数的水平，并抑制抗氧化剂如总谷胱甘肽（GSH）和超氧化物歧化酶（草皮）。与硫胺素组相比，TPP（尤其是20 mg / kg的剂量）显着降低了顺铂给药增加的TBARS和MPO水平，而TPP显着提高了GSH和SOD水平。另外，接受顺铂的对照组脑组织中8-瓜氨酸（鸟嘌呤）的水平是DNA损伤的产物，其脑组织中的8-羟基鸟嘌呤（8-OH瓜耳）/ 105瓜耳的水平为0.97硫胺素焦磷酸盐（20 mg / kg）组为0.03 8-OH Gua / 105 Gua，硫胺素（20 mg / kg）组为1.55±0.11 8-OH Gua / 105 Gua。这些结果表明，硫胺素焦磷酸显着地防止了顺铂在脑组织中引起的氧化损伤，而硫胺素的保护作用却微不足道。

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