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首页> 外文期刊>Zoonoses and Public Health >Isolation of Campylobacter from feral swine (Sus scrofa) on the ranch associated with the 2006 Escherichia coli O157:H7 spinach outbreak investigation in California.
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Isolation of Campylobacter from feral swine (Sus scrofa) on the ranch associated with the 2006 Escherichia coli O157:H7 spinach outbreak investigation in California.

机译：在与2006年加利福尼亚州大肠杆菌（O157：H7）菠菜暴发调查相关的牧场上，从野猪（Sus scrofa ）中分离出弯曲杆菌（Campylobacter ）。

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We report the isolation of Campylobacter species from the same population of feral swine that was investigated in San Benito County, California, during the 2006 spinach-related Escherichia coli O157:H7 outbreak. This is the first survey of Campylobacter in a free-ranging feral swine population in the United States. Campylobacter species were cultured from buccal and rectal-anal swabs, colonic faeces and tonsils using a combination of selective enrichment and antibiotic-free membrane filtration methods. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS, Bruker Daltonics, Inc., Billerica, MA, USA) was used to identify species followed by confirmatory multiplex PCR or 16S rRNA sequencing. Genetic relatedness of Campylobacter jejuni and Campylobacter coli strains was determined by multilocus sequence typing (MLST) and porA allele sequencing. Altogether, 12 (40%) of 30 feral swine gastrointestinal and oral cavity specimens were positive, and six species were isolated: Campylobacter coli, Campylobacter fetus, Campylobacter hyointestinalsis, Campylobacter jejuni, Campylobacter lanienae and Campylobacter sputorum. Campylobacter jejuni subtypes were closely related to MLST sequence type 21 (ST-21) and had identical porA sequences. Campylobacter coli subtypes were unrelated to isolates in the pubMLST/porA database. This feral swine population lived in close association with a "grassfed" beef cattle herd adjacent to spinach and other leafy green row crop fields. The findings underscore the importance of protecting raw vegetable crops from faecal contamination by wild or feral animals. The study also illustrates a potential risk of Campylobacter exposure for hunters during handling and processing of wild swine meat. copyright 2012 Blackwell Verlag GmbH.
机译：我们报告了在2006年与菠菜相关的大肠杆菌 O157：H7爆发期间，从加利福尼亚圣贝尼托县调查的同一批野猪中分离出<弯曲菌>弯曲杆菌的种。 。这是在美国自由放养的野猪种群中对弯曲杆菌的首次调查。使用选择性富集和无抗生素膜过滤方法的组合，从颊和直肠肛门拭子，结肠粪便和扁桃体中培养 Campylobacter 。使用基质辅助激光解吸电离飞行时间质谱仪（MALDI-TOF-MS，美国布鲁克林道尔顿公司，马萨诸塞州比勒里卡，美国）来鉴定物种，然后进行确认性多重PCR或16S rRNA测序。通过多基因座序列分型（MLST）和 po rA等位基因测序确定空肠弯曲杆菌和大肠弯曲杆菌的遗传相关性。总共30头野生猪胃肠道和口腔标本中有12例（40％）呈阳性，并分离出6种： Campylobacter coli ， Campylobacter fetus ， Campylobacter hyointestinalsis ，空肠弯曲杆菌，兰氏弯曲杆菌和 sputorum 。空肠弯曲杆菌亚型与MLST序列类型21（ST-21）密切相关，并且具有相同的 porA 序列。 弯曲杆菌亚型与pubMLST / po rA数据库中的分离株无关。这种野猪种群与邻近菠菜和其他绿叶农作物田的“草食”肉牛群密切相关。这些发现强调了保护原始蔬菜作物免受野生或野生动物粪便污染的重要性。这项研究还说明了猎人在处理和加工野生猪肉过程中可能暴露出弯曲杆菌的潜在风险。版权所有2012 Blackwell Verlag GmbH。

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