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首页> 外文期刊>Zoological Science >Intraspecific differentiation in the Japanese brown frog Rana japonicainferred from mitochondrial DNA sequences of the cytochrome b gene
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Intraspecific differentiation in the Japanese brown frog Rana japonicainferred from mitochondrial DNA sequences of the cytochrome b gene

机译：从细胞色素b基因的线粒体DNA序列推断日本褐蛙林蛙种内分化

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摘要

Intraspecific differentiation of the Japanese brown frog Rana japonica was investigated by analyzing nucleotide sequences of the mitochondrial cytochrome b (cyt b) gene in order to clarify phylogenetic relationships among three population groups known to exist in this species. The nucleotide sequences of 447 base pair (bp) segments were determined by the PCR-direct sequencing method on 31 individuals from 14 populations of R. japonica from Honshu, and phylogenetic trees were constructed by the UPGMA and NJ methods using Rana catesbeiana as an outgroup. A sequence alignment provided 92 variable sites (15 corresponded to the first codon position, three to the second, and 74 to the third), and 19 haplotypes were identified from 31 frogs. The sequence divergences were 0.22-2.50% ((x) over bar = 0.65%) within populations, 0.22 similar to 12.02% ((x) over bar = 7.34%) between populations, and 23.59 similar to 27.89% ((x) over bar = 26.19%) between R. japonica and R. catesbeiana. Although many nucleotide substitutions were silent mutations, 12 amino acid replacements were found to occur within R, japonica. A high frequency of transitions relative to transversions was observed within R, japonica. The present nucleotide sequence data showed that the eastern and western groups of R. japonica were considerably differentiated to each other, and that the Akita population of the northwestern group was evidently derived from the eastern group, but the Nakajo and Izumozaki populations of the northwestern group diverged considerably from each of the eastern and the western groups.
机译：通过分析线粒体细胞色素b（cyt b）基因的核苷酸序列，研究了日本褐蛙林蛙的种内分化，以阐明该物种中已知的三个种群之间的系统发育关系。采用PCR-直接测序方法，对本州14个粳稻种群的31个个体进行了PCR扩增，确定了447个碱基对的碱基对，并通过UPGMA和NJ方法构建了系统树。 。序列比对提供了92个可变位点（15个对应于第一个密码子位置，3个对应于第二个密码子位点，74个对应于第三个密码子位点），并且从31只青蛙中鉴定出19个单倍型。群体内的序列差异为0.22-2.50％（bar之上的（x）= 0.65％），群体之间的序列差异为0.22类似于12.02％（bar之上的（x）= 7.34％），以及人群之间的序列差异为23.59，类似于27.89％（bar之上的（x）条形粳稻和Catesbeiana之间的条形图= 26.19％）。尽管许多核苷酸取代是沉默突变，但发现日本粳稻中发生了12个氨基酸取代。在日本粳稻中观察到相对于转化的高频率转化。目前的核苷酸序列数据表明，日本粳稻的东部和西部群体之间有很大的差异，西北组的秋田种群显然来自东部，而西北组的中城和泉崎种群东方和西方各族之间的分歧很大。

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