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首页> 外文期刊>Zoological Science >Identification of Xenopus cortactin: Two isoforms of the transcript andmultiple forms of the protein
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Identification of Xenopus cortactin: Two isoforms of the transcript andmultiple forms of the protein

机译：爪蟾cortactin的鉴定：转录本的两个亚型和该蛋白的多种形式

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Cortactin was initially identified as a substrate for Src tyrosine kinase. It interacts with the filamentous actin in the cell cortex through the tandem repeats of 37-amino acid. In this report, we describe the identification of a Xenopus homolog of cortactin. The deduced amino acid sequence shares over 70% identity with human, mouse, and chicken cortactin. Northern and Western blot analyses revealed that Xenopus cortactin is widely expressed in Xenopus tissues. Analysis of the transcripts using polymerase chain reaction revealed two isoforms being different in the number of the tandem repeats. The major isoform has 6.5 tandem repeats but the minor one has 5.5 tandem repeats. As the sixth repeat, which is missed in the minor isoform, is encoded by a single exon flanked by introns on both sides, these two isoforms are likely to be generated by alternative splicing. We propose that cortactin regulates the construction of actin cytoskeleton by altering the number of its tandem repeats.
机译：最初确定Cortactin是Src酪氨酸激酶的底物。它通过37个氨基酸的串联重复序列与细胞皮质中的丝状肌动蛋白相互作用。在这份报告中，我们描述了cortactin的非洲爪蟾同系物的鉴定。推导的氨基酸序列与人，小鼠和鸡的cortactin具有超过70％的同一性。 Northern和Western印迹分析表明，爪蟾cortactin在爪蟾组织中广泛表达。使用聚合酶链反应对转录本进行分析，发现两个同工型的串联重复数不同。主要同工型具有6.5个串联重复序列，而次要同工型具有5.5个串联重复序列。由于次要亚型中缺少的第六个重复序列由两侧均含内含子的单个外显子编码，因此这两个亚型很可能是通过选择性剪接产生的。我们建议，cortactin通过改变其串联重复序列的数量来调节肌动蛋白细胞骨架的构建。

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