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首页> 外文期刊>Zoological Science >Influence of Recovery Method and Centrifugation on Epididymal Sperm from Collared Peccaries (Pecari tajacu Linnaeus, 1758)
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Influence of Recovery Method and Centrifugation on Epididymal Sperm from Collared Peccaries (Pecari tajacu Linnaeus, 1758)

机译：恢复方法和离心分离对衣领附睾附睾精子的影响（Pecari tajacu Linnaeus，1758）

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摘要

In order to establish protocols for gamete recovery from accidentally killed wild animals, or to take advantage of those slaughtered by captive breeders, we assess the influence of two methods on the recovery of epididymal sperm from collared peccaries, and verify the effect of centrifugation on such gametes. Genitalia from nine animals were used. For each animal, one epididymis was processed by flotation and the other was processed by retrograde flushing, both using a buffered media based on Tris. Following recovery, sperm were evaluated for motility, vigor, viability, functional membrane integrity, and morphology. A 1-mL aliquot of each sample was centrifuged, the supernatant removed, and the pellet suspended and evaluated as fresh samples. The sperm characteristics did not differ between the samples collected by flotation or retrograde flushing (P < 0.05). Centrifugation promoted an increase in head and tail defects, thus reducing the percentage of viable sperm (P < 0.05). No other parameter assessed for both methods was affected by centrifugation. In conclusion, epididymal sperm from collared peccaries can be efficiently collected through flotation or retrograde flushing, but not when either is followed by centrifugation.
机译：为了建立从意外杀死的野生动物中进行配子恢复的协议，或利用被圈养繁殖者屠宰的动物，我们评估了两种方法对从领兽角的附睾精子中恢复的影响，并验证了离心对此类动物的影响。配子。使用了来自九只动物的生殖器。对于每只动物，一只附睾通过浮选进行处理，另一只通过逆行冲洗进行处理，两者均使用基于Tris的缓冲液进行。恢复后，评估精子的运动能力，活力，生存能力，功能性膜完整性和形态。将每个样品的1 mL等分试样离心，除去上清液，将沉淀物悬浮并评估为新鲜样品。通过浮选或逆行冲洗收集的样品之间的精子特性没有差异（P <0.05）。离心促进头和尾部缺陷的增加，从而降低了活精子的百分比（P <0.05）。两种方法均未评估其他参数受离心作用的影响。总之，可以通过浮选或逆行冲洗有效地收集领领兽的附睾精子，但离心分离后则不能。

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