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Expression and Syntenic Analyses of Four nanos Genes in Medaka

机译:Medaka中四个纳米基因的表达和同义分析

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The gene nanos is essential for germ cell development. Although its functions and expression have been investigated in the mouse, nanos genes have yet to be well characterized in other vertebrates. Based on similarity and a syntenic analysis of nanos, we have identified four different nanos in the genome of medaka (Oryzias latipes). Nanos1 is duplicated in teleost fish genomes and named nanos1a and nanos1b. Of all medaka nanos, nanos3 is well conserved in terms of expression and synteny. In contrast to a previous study on mice, nanos2 expression was not detected in the gonads at early stages of sex differentiation; however, both oogonia and spermatogonia in adult gonads exhibit nanos2 expression. Nanos1a and 1b are both expressed in the developing brain, consistent with the expression of nanos1 in mice. In the gonads, nanos1a is expressed in the somatic cells surrounding oocytes and spermatocytes, whereas expression of nanos1b is not detectable in the gonads by in-situ hybridization. These results suggest common and distinct functions of nanos genes among vertebrates.
机译:纳米基因对于生殖细胞发育至关重要。尽管已经在小鼠中研究了它的功能和表达,但是在其他脊椎动物中尚未很好地表征nanos基因。基于相似性和对纳米的同义分析,我们在青aka(Oryzias latipes)的基因组中鉴定出四个不同的纳米。 Nanos1在硬骨鱼基因组中重复存在,分别命名为nanos1a和nanos1b。在所有medaka nanos中,nanos3在表达和同义性方面都非常保守。与先前的小鼠研究相反,在性别分化的早期阶段,在性腺中未检测到nanos2表达。然而,成年性腺中的卵小球藻和精原细胞均表现出nanos2表达。 Nanos1a和1b都在发育中的大脑中表达,与nanos1在小鼠中的表达一致。在性腺中,nanos1a在卵母细胞和精母细胞周围的体细胞中表达,而通过原位杂交无法在性腺中检测到nanos1b的表达。这些结果表明,在脊椎动物中,纳米基因具有共同而独特的功能。

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