...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Vaccine >Derivation of attenuated porcine epidemic diarrhea virus (PEDV) as vaccine candidate
【24h】

Derivation of attenuated porcine epidemic diarrhea virus (PEDV) as vaccine candidate

机译：减毒猪流行性腹泻病毒（PEDV）的衍生疫苗

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The field isolate of porcine epidemic diarrhea virus (PEDV) was serially passaged in Vero cells. The cell passaged PEDV, designated KPEDV-9, was tested for its pathogenicity in neonatal pigs, immunogenicity and safety in the pregnant sows. 53 and 11piglets, respectively were i.m. inoculated with 106.0 and 107.0 TCID50/ml of cell adapted PEDV. None of thes piglets had diarrhoea or symptoms related to PEDV. Eight piglets were infected orally with 10 ml of virus stock which contained virus at 108.0 TCID50/ml and were artificially fed with dairy milk. Three pigs showed signs of anorexia and diarrhoea in two or three days, but these signs were transient. One of 3 pregnant sows was inoculated with the attenuated virus and showed increased immune responses by ELISA. In addition, one group of 10 and two groups of 5 delivered piglets were shown to be protected from challenge with wild type PEDV when compared with the challenge of one group of 10 and one group of 5 control piglets. The safety test involving 63 pregnant sows indicated that all inoculated animals farrowed the average numbers of litters of piglets. It is concluded that the attenuated virus derived from serial passage could be applied as a vaccine for suckling piglets against PEDV.
机译：猪流行性腹泻病毒（PEDV）的野外分离株在Vero细胞中连续传代。测试了通过细胞传代的PEDV（称为KPEDV-9）在新生猪中的致病性，在怀孕母猪中的免疫原性和安全性。 53只和11只小猪分别是上午接种106.0和107.0 TCID50 / ml的细胞适应性PEDV。这些小猪均无腹泻或与PEDV相关的症状。八头仔猪经口感染10毫升病毒原种，其中含有108.0 TCID50 / ml的病毒，并人工喂养乳汁。三到三天的猪表现出厌食和腹泻的迹象，但是这些迹象是短暂的。用减毒病毒接种三只怀孕母猪中的一只，并通过ELISA显示增强的免疫反应。另外，与一组10只和一组5只对照仔猪的攻击相比，一组10只和两组5只分娩的小猪显示出免受野生型PEDV攻击的保护。涉及63头怀孕母猪的安全性测试表明，所有接种的动物均产仔猪的平均产仔数。结论是，来自连续传代的减毒病毒可以用作针对PEDV的乳猪的疫苗。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号