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首页> 外文期刊>Vaccine >Bacterial ghosts as a delivery system for zona pellucida-2 fertility control vaccines for brushtail possums (Trichosurus vulpecula)
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Bacterial ghosts as a delivery system for zona pellucida-2 fertility control vaccines for brushtail possums (Trichosurus vulpecula)

机译：细菌幽灵作为带状负鼠（Trichosurus vulpecula）的透明带2生育力控制疫苗的递送系统

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The introduced brushtail possum is a serious pest in New Zealand and there is much interest in the development of an immunocontraceptive vaccine for population control. Immunisation of female possums against recombinant possum zona pellucida protein-2 (ZP2) is known to reduce embryo production by 72-75% but successful development of fertility control will depend on a delivery system that is effective for field use. Bacterial ghost vaccine technology is a promising system to formulate a non-living vaccine for bait or aerosol delivery. The N-terminal (amino acid residues 41-316, ZP2N) and C- terminal (amino acid residues 308-636, ZP2C) regions of possum ZP2 were fused to maltose-binding protein and expressed in the periplasmic space of Escherichia coli NM522 bacterial ghosts. Female possums (n = 20 per treatment group) were immunised with 20 mg of either plain ghosts, ZP2N ghosts, or ZP2C ghosts in phosphate-buffered saline applied to the nostrils and eyes (nasal/conjunctival mucosa) at weeks 0, 2 and 4. Effects of immunisation on fertility were assessed following superovulation and artificial insemination. Both constructs evoked humoral (antibody) and cell- mediated immune responses in possums and significantly fewer eggs were fertilised in females immunised against ZP2C ghosts. Results in this study indicate that bacterial ghosts containing possum ZP antigens can reduce possum fertility when delivered by mucosal immunisation and offer a promising delivery system for fertility control of wild possum populations.
机译：引入的猪尾负鼠是新西兰的一种严重害虫，人们对开发用于控制种群的免疫避孕疫苗非常感兴趣。雌性负鼠针对重组负鼠透明带蛋白2（ZP2）的免疫可将胚胎产量降低72-75％，但成功进行生育控制的方法将取决于对田间有效使用的递送系统。细菌幽灵疫苗技术是一种有前途的系统，可用于配制用于诱饵或气雾剂递送的无生命疫苗。负鼠ZP2的N末端（氨基酸残基41-316，ZP2N）和C末端（氨基酸残基308-636，ZP2C）与麦芽糖结合蛋白融合，并在大肠杆菌NM522细菌的周质空间表达鬼在第0、2和4周时，将雌性负鼠（每个治疗组n = 20）用磷酸盐缓冲液中的20 mg普通鬼魂，ZP2N鬼魂或ZP2C鬼魂进行免疫，以免疫鼻孔和眼（鼻/结膜黏膜）在超排卵和人工授精后评估免疫对生育力的影响。两种构建体均引起负鼠的体液（抗体）和细胞介导的免疫反应，并且在针对ZP2C幽灵免疫的雌性中受精的卵明显减少。这项研究的结果表明，含有负鼠ZP抗原的细菌幽灵在通过粘膜免疫进行递送时可以降低负鼠的繁殖力，并为控制野生负鼠种群的繁殖力提供了有希望的递送系统。

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