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首页> 外文期刊>Vaccine >Outer membrane vesicles as acellular vaccine against pertussis.
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Outer membrane vesicles as acellular vaccine against pertussis.

机译：外膜囊泡作为抗百日咳的脱细胞疫苗。

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In this study the development and evaluation of outer membrane vesicles (OMVs) obtained from Bordetella pertussis as vaccines against pertussis disease is described. SDS-PAGE, immunoblot techniques and gel electrophoresis associated to tandem mass spectrometry were used to describe the composition of the OMVs obtained from B. pertussis Tohama CIP 8132 strain. These techniques revealed the presence of the main well-known pertussis surface immunogens in the OMVs such as pertactin, adenylate cyclase-haemolysin, pertussis toxin, as well as the lipo-oligosaccharide (LOS). A total of 43 proteins were identified by mass spectrometry. Some of them were predicted to have outer membrane or periplasmic location and the others with cytoplasmic or unknown location. The characterized pertussis OMVs were used in murine B. pertussis intranasal (i.n.) challenge model to examine their protective capacity when delivered by different routes. Killed detoxified whole-cell B. pertussis bacteria were used as reference. For intraperitoneal (i.p.) immunization, aluminum hydroxide was used as adjuvant. Since i.n. treatment with OMVs as well as killed whole-cell bacteria enhanced markers of innate immune response such as TNFalpha, IL-6 and CCL20, i.n. immunizations were performed with no adjuvant added. Immunized BALB/c mice were intranasally challenged with sublethal doses of B. pertussis. Significant differences between immunized animals and the PBS treated group were observed (p0.001). Adequate elimination rates (p0.005) were observed in mice immunized either with OMV or whole-cell bacteria. Comparable results were obtained with both types of immunization route. In view to their capacity to induce airways innate and protective immunity in the mouse model, OMVs obtained from B pertussis are candidates to be used to protect against pertussis.
机译：在这项研究中，描述了从百日咳博德特氏菌获得的外膜囊泡（OMV）作为百日咳疾病疫苗的开发和评估。 SDS-PAGE，免疫印迹技术和与串联质谱法相关的凝胶电泳被用来描述从百日咳博德特氏菌Tohama CIP 8132菌株获得的OMV的组成。这些技术揭示了在OMV中存在主要的众所周知的百日咳表面免疫原，例如百日咳杆菌粘附素，腺苷酸环化酶-溶血素，百日咳毒素和脂寡糖（LOS）。通过质谱鉴定总共43种蛋白质。预测其中一些具有外膜或周质位置，而其他一些具有细胞质或未知位置。将特征化的百日咳OMV用于鼠百日咳博德特氏菌鼻内（i.n.）攻击模型，以检查通过不同途径递送时的保护能力。将杀死的解毒的全细胞百日咳博德特氏菌用作参考。为了腹膜内（i.p.）免疫，使用氢氧化铝作为佐剂。自从用OMV以及杀死的全细胞细菌进行治疗可增强先天性免疫应答的标记物，例如TNFα，IL-6和CCL20，在不添加佐剂的情况下进行免疫。用亚致死剂量的百日咳博德特氏菌对免疫的BALB / c小鼠进行鼻内攻击。观察到免疫动物和PBS处理组之间的显着差异（p <0.001）。在用OMV或全细胞细菌免疫的小鼠中观察到了足够的消除率（p <0.005）。两种免疫途径均获得了可比的结果。考虑到它们在小鼠模型中诱导气道先天性和保护性免疫的能力，从百日咳博德特氏菌获得的OMVs可以用于预防百日咳。

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