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首页> 外文期刊>Vaccine >Characterization of meningococcal serogroup B outer membrane vesicle vaccines from strain 44/76 after growth in different media.
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Characterization of meningococcal serogroup B outer membrane vesicle vaccines from strain 44/76 after growth in different media.

机译：在不同培养基中生长后，来自菌株44/76的脑膜炎球菌血清群B外膜囊泡疫苗的表征。

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In this study, we evaluated the effect of the growth medium on the composition and immunogenicity of meningococcal outer membrane vesicle (OMV) vaccines after cultivation of the Norwegian serogroup B 44/76 vaccine strain in either Frantz' or modified Catlin-6 media (MC.6M). Differential proteomic analysis revealed that 97% of the OMV proteins maintained the same levels in the two preparations. However, a number of differentially expressed proteins, including TdfH, OpcA, OMP NMB0088, hypothetical NMB2134, lipoprotein NMB1126/1164 and NspA, increased significantly in OMVs produced from bacteria grown in the MC.6M. Together with increased lipopolysaccharide levels, the increased expression of these proteins was associated with significantly higher serum bactericidal titres in mice immunized with the MC.6M OMV vaccine. The high resolution two-dimensional separation of the OMVs on a large-format gel across a pH range of 3-11 resolved around 2000 protein spots from which 75 proteins were identified by mass spectrometry.
机译：在这项研究中，我们评估了在Frantz'或改良Catlin-6培养基（MC）中培养挪威B 44/76血清群疫苗株后，生长培养基对脑膜炎球菌外膜囊泡（OMV）疫苗的组成和免疫原性的影响。 .6M）。差异蛋白质组学分析表明，两种制剂中97％的OMV蛋白保持相同水平。但是，在MC.6M中生长的细菌产生的OMV中，许多差异表达的蛋白质（包括TdfH，Opcc，OMP NMB0088，假定的NMB2134，脂蛋白NMB1126 / 1164和NspA）显着增加。与增加的脂多糖水平一起，这些蛋白的表达增加与用MC.6M OMV疫苗免疫的小鼠的血清杀菌滴度显着相关。在3-11 pH范围内，大幅面凝胶上OMV的高分辨率二维分离可解决2000个蛋白质斑点，其中质谱鉴定出75种蛋白质。

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