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首页> 外文期刊>Vaccine >GM-CSF enhances protective immunity to cottontail rabbit papillomavirus E8 genetic vaccination in rabbits
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GM-CSF enhances protective immunity to cottontail rabbit papillomavirus E8 genetic vaccination in rabbits

机译：GM-CSF增强了对棉尾兔乳头瘤病毒E8基因疫苗的保护性免疫

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We have reported previously that cottontail rabbit papillomavirus (CRPV) E8 gene immunization induced strong protection against virus challenge. In this study, we primed E8 gene vaccination with mouse granulocyte-macrophage colony-stimulating factor (mGM-CSF), a cytokine that induces differentiation and local recruitment of professional antigen-presenting cells. EIII/JC inbred rabbits were divided into four groups receiving vaccinations with the following constructs: mGM-CSF plus E8, mGM-CSF only, E8 only and vector only. After three immunizations at intervals of 3 weeks, rabbits were challenged with viral DNA at six scarified sites. Papillomas grew on all vaccinated rabbits 4 weeks after inoculation. At week 5, papillomas on four rabbits of mGM-CSF plus E8 and one of E8 only rabbits began to regress. At week 11, all the papillomas on rabbits in the GM-CSF plus E8 vaccination group regressed (regression rate = 100%); regression rates of the mGM-CSF only and E8 only vaccination groups were 50 and 25%, respectively. All papillomas on the vector immunized rabbits remained persistent until the end of the experiment (0%). Antibodies to mGM-CSF were detected in rabbit serum by Western blot. Rabbits vaccinated with E8 plus mGM-CSF or E8 only group had positive Delayed-type hypersensitivity (DTH) skin test to different E8 peptides. These results demonstrated that mGM-CSF could enhance the effects of E8 immunization in rabbits to CRPV infection through cell-mediated immune responses
机译：以前我们已经报道过，棉尾兔乳头瘤病毒（CRPV）E8基因免疫诱导了针对病毒攻击的强大保护作用。在这项研究中，我们用小鼠粒细胞-巨噬细胞集落刺激因子（mGM-CSF）引发了E8基因疫苗接种，mGM-CSF是一种诱导专业抗原呈递细胞分化和局部募集的细胞因子。将EIII / JC近交系兔分为以下四组，分别接种以下疫苗：mGM-CSF加E8，仅mGM-CSF，仅E8和仅载体。每隔3周进行3次免疫后，在六个疤痕处用病毒DNA攻击兔子。接种后4周，所有接种的兔子均出现乳头状瘤。在第5周，对四只mGM-CSF加E8的兔子和一只仅E8的兔子的乳头状瘤开始消退。在第11周，GM-CSF加E8疫苗接种组的所有兔乳头状瘤均消退（消退率= 100％）;仅mGM-CSF和仅E8疫苗接种组的回归率分别为50％和25％。经载体免疫的兔子上的所有乳头状瘤均持续存在直至实验结束（0％）。通过Western印迹在兔血清中检测到针对mGM-CSF的抗体。接种了E8加mGM-CSF或仅E8的组的兔子对不同的E8肽的阳性迟发型超敏反应（DTH）皮肤试验阳性。这些结果表明，mGM-CSF可以通过细胞介导的免疫反应增强兔E8免疫CRPV感染的效果。

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