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首页> 外文期刊>Vox Sanguinis: International Journal of Blood Transfusion and Immunohaematology >Six years' experience of using the BacT/ALERT system to screen all platelet concentrates, and additional testing of outdated platelet concentrates to estimate the frequency of false-negative results.
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Six years' experience of using the BacT/ALERT system to screen all platelet concentrates, and additional testing of outdated platelet concentrates to estimate the frequency of false-negative results.

机译：使用BacT / ALERT系统筛查所有血小板浓缩液的六年经验，以及对过时的血小板浓缩液的其他测试以估计假阴性结果的频率。

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Background and Objectives Approximately 1 in every 2000 units of platelets is contaminated with bacteria. The BacT/ALERT automated blood culture system can be used to screen platelet concentrates (PCs) for bacterial contamination. Materials and Methods Data were collected from May 1998 until May 2004. The number of PCs tested during this period was 36 896, most of which were produced from pools of four buffy-coats. On the day following blood collection or platelet apheresis, a 5-10 ml sample of the PC was aseptically transferred to a BacT/ALERT culture bottle for detection of aerobic bacteria. The sample was monitored for bacterial growth during the entire storage period of the PC (6.5 days). When a positive signal was generated, the culture bottle, the PC and the erythrocyte concentrates were tested for bacterial growth. In order to determine the frequency of false-negative BacT/ALERT signals, 1061 outdated PCs were tested during the period from May 2002 to May 2004. Results Eighty-eight positive signals were detected by the BacT/ALERT system, of which 12 were interpreted as truly positive. Fourteen signals were interpreted as truly false positive. Thirty-three signals were interpreted to be probably false positive. Two of 1061 outdated units tested positive, and Bacillus spp. and Staphylococcus epidermidis, respectively, were isolated from these PCs. Conclusions Between 0.03% and 0.12% of the PCs were contaminated with bacteria. BacT/ALERT is an efficient tool for monitoring PCs for bacterial contamination; however, it is important to realize that false-negative results may occur.
机译：背景和目的每2000个血小板中约有1个被细菌污染。 BacT / ALERT自动血液培养系统可用于筛查浓缩血小板（PC）的细菌污染。材料和方法数据收集自1998年5月至2004年5月。在此期间测试的PC数量为36 896，其中大部分是由四层血沉棕黄层组成的。在采血或血小板单采的第二天，将5-10毫升的PC样品无菌转移到BacT / ALERT培养瓶中以检测需氧细菌。在PC的整个存储期间（6.5天）内监视样品的细菌生长。当产生阳性信号时，测试培养瓶，PC和浓缩红细胞的细菌生长。为了确定BacT / ALERT假阴性信号的频率，在2002年5月至2004年5月期间测试了1061台过时的PC。结果BacT / ALERT系统检测到88个阳性信号，其中12个被解释真正的积极。十四个信号被解释为真正的假阳性。 33个信号可能被解释为假阳性。 1061个过时的单元中有两个测试为阳性，芽孢杆菌属（Bacillus spp）。从这些PC中分别分离出表皮葡萄球菌和表皮葡萄球菌。结论0.03％至0.12％的PC被细菌污染。 BacT / ALERT是监视PC上细菌污染的有效工具。但是，重要的是要意识到可能会出现假阴性结果。

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