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首页> 外文期刊>Turkish Journal of Agriculture & Forestry >Transferability of SSR markers from distantly related legumes to Glycyrrhiza species
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Transferability of SSR markers from distantly related legumes to Glycyrrhiza species

机译：SSR标记从远缘豆类到甘草种的转移性

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摘要

Licorice (Glycyrrhiza spp.) is an important medicinal plant and its distribution of natural habitats has been shrinking day by day due to extensive collection. Genetic diversity identification will likely assist in conserving the different Glycyrrhizaspecies. A total of 127 simple sequence repeat (SSR) markers from Medicago truncatula Gaertn., Phaseolus vulgaris L., and deer arietinum L. were used and 26 of them were amplified on Glycyrrhiza genomes. The highest transferability rate (33%) was obtained from M. truncatula markers, while the highest genetic diversity values were obtained in P. vulgaris markers. The markers BM153 and PV-ag004 from P. vulgaris had the largest polymorphism information content. The amplified primers were also used to identify genetic diversity among three Glycyrrhiza species. The gene diversity values among Glycyrrhiza species appeared to be similar; however, donor species mostly had lower diversity values than those of Glycyrrhiza species. Additionally, the genetic analysis showed that G. flavescens Boiss. subsp. flavescens is more distantly related to species G. glabra L. var. glandulifera and G. echinata L. The number of alleles was mostly higher than in the donor species, possibly proposing a multiallelic and/or polyploid structure in Glycyrrhiza species.
机译：甘草（甘草）是一种重要的药用植物，由于广泛的采集，其自然栖息地的分布日益缩小。遗传多样性鉴定将可能有助于保护不同的甘草种。总共使用了来自Medicago truncatula Gaertn。，菜豆和菜鹿的127个简单序列重复（SSR）标记，并在甘草基因组上扩增了其中的26个。从truncatula标记获得最高的可转移性（33％），而在寻常型标记中获得最高的遗传多样性。来自寻常型假单胞菌的标记物BM153和PV-ag004具有最大的多态性信息含量。扩增的引物还用于鉴定三种甘草种之间的遗传多样性。甘草种之间的基因多样性值似乎是相似的。然而，施主物种的多样性值大多低于甘草物种。另外，遗传分析表明黄萎病菌Boiss。亚种flavescens与G. glabra L. var。种的关系更远。 glandulifera和G. echinata L.等位基因的数量大多高于供体物种，可能在甘草中提出了多等位基因和/或多倍体结构。

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