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首页> 外文期刊>Turkish journal of biology >Production of biosurfactant by Pseudomonas spp. isolated from industrial waste in Turkey
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Production of biosurfactant by Pseudomonas spp. isolated from industrial waste in Turkey

机译：假单胞菌属物种生产生物表面活性剂。从土耳其的工业废物中分离出来

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摘要

In this study, 26 Pseudomonas spp. were isolated from a stream polluted by factory waste and from petroleum-contaminated soil. The surface tension (ST) of the cultures was used as a criterion for the primary isolation of biosurfactant-producing bacteria. Biosurfactant production was quantified by ST reduction, critical micelle concentration (CMC), emulsification capacity (EC), and cell surface hydrophobicity (CSH). Two of the isolates, P. aeruginosa 78 and 99, produced rhamnolipid biosurfactant. The strains started rhamnolipid production in the logarithmic phase. They decreased the ST of the culture from 73 dyne/cm2 to 29 and 33 dyne/cm2, and the CMC of produced rhamnolipids were 115 and 130 mg/L, respectively. P. aeruginosa 78 and 99 strains emulsified benzene and n-hexane at the highest rates, and the surfaces of these strains were 73% and 65% and 62% and 72% more hydrophobic for benzene and toluene, respectively.
机译：在这项研究中，26假单胞菌属。从工厂废物污染的溪流和石油污染的土壤中分离出来。培养物的表面张力（ST）被用作初步分离产生生物表面活性剂的细菌的标准。通过ST还原，临界胶束浓度（CMC），乳化能力（EC）和细胞表面疏水性（CSH）量化生物表面活性剂的产生。两种分离株，铜绿假单胞菌78和99，产生鼠李糖脂生物表面活性剂。菌株在对数期开始鼠李糖脂的产生。他们将培养物的ST从73达因/平方厘米降低到29达因/平方厘米和33达因/平方厘米，生产的鼠李糖脂的CMC分别为115和130 mg / L。铜绿假单胞菌78和99菌株以最高的速率乳化苯和正己烷，这些菌株的表面对苯和甲苯的疏水性分别提高了73％，6​​5％和62％和72％。

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