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首页> 外文期刊>DNA and Cell Biology >Interaction of Safranal and Picrocrocin with ctDNA and Their Preferential Mechanisms of Binding to GC- and AT-Rich Oligonucleotides
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Interaction of Safranal and Picrocrocin with ctDNA and Their Preferential Mechanisms of Binding to GC- and AT-Rich Oligonucleotides

机译：Sactnal和青霉素与ctDNA的相互作用及其与富含GC和AT的寡核苷酸结合的优先机制

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摘要

Saffron (Crocus sativus L.) shows different biological properties, some of which relate to its special components including safranal (deglycosylated picrocrocin). In our previous study on the mechanism of saffron functions, interaction of saffron carotenoids with DNA and oligonucleotides was reported. The present study aimed to investigate the interaction of safranal with calf thymus DNA (ctDNA), oligo(GC)(15), and oligo(AT)(15) in comparison with picrocrocin. Circular dichroism (CD) and fluorometric results show the nonintercalative/minor groove binding of the mentioned ligands to ctDNA. The CD plots indicate that picrocrocin interacts with DNA and oligonucleotides at lower concentrations than safranal does, which is accompanied by B- to C-DNA transition. At higher concentrations, it induces more conformational changes, that is, unstacking of DNA and oligonucleotides bases, the same as saffron carotenoids. However, similar results are obtained for safranal interaction with ctDNA (56% AT) and oligo(AT)(15), but after adding safranal (with a free aldehyde group) to oligo(GC)(15), the CD spectra show some changes (two positive and a displaced negative peaks) that are characteristics of triple-helix DNA. Thus, the mechanism for B- to H-DNA transition, due to the interaction of safranal with GC-rich sequences, is presented here.
机译：藏红花（Crocus sativus L.）具有不同的生物学特性，其中一些与它的特殊成分有关，包括sa（去糖基化的多柔红霉素）。在我们先前关于藏红花功能机制的研究中，曾报道过藏红花类胡萝卜素与DNA和寡核苷酸的相互作用。本研究旨在调查aimed与小牛胸腺DNA（ctDNA），oligo（GC）（15）和oligo（AT）（15）的相互作用。圆二色性（CD）和荧光分析结果表明，上述配体与ctDNA的非插入/小沟结合。 CD曲线表明，微青霉素与DNA和寡核苷酸的相互作用浓度低于Safranal的浓度，并伴有B-向C-DNA的转变。在较高的浓度下，它会诱导更多的构象变化，即与藏红花类胡萝卜素一样，DNA和寡核苷酸碱基的解叠。但是，与ctDNA（56％AT）和oligo（AT）（15）的人胚芽相互作用也获得了相似的结果，但是在向寡聚（GC）（15）添加人胚芽（具有游离醛基）后，CD谱显示出一些改变（两个正峰和一个置换负峰），这是三螺旋DNA的特征。因此，在这里介绍了由于safranal与富含GC的序列之间的相互作用，从B向H-DNA过渡的机制。

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