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首页> 外文期刊>Chemistry & biology >A complete gene cluster from Streptomyces nanchangensis NS3226 encoding biosynthesis of the polyether ionophore nanchangmycin
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A complete gene cluster from Streptomyces nanchangensis NS3226 encoding biosynthesis of the polyether ionophore nanchangmycin

机译：南昌链霉菌NS3226的完整基因簇，编码聚醚离子载体南昌霉素的生物合成

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The PKS genes for biosynthesis of the polyether nanchangmycin are organized to encode two sets of proteins (six and seven ORFs, respectively), but are separated by independent ORFs that encode an epimerase, epoxidase, and epoxide hydrolase, and, notably, an independent ACP. One of the PIKS modules lacks a corresponding ACP. We propose that the process of oxidative cyclization to form the polyether structure occurs when the polyketide chain is still anchored on the independent ACP before release. 4-0-methyl-L-rhodinose biosynthesis and its transglycosylation involve four putative genes, and regulation of nanchangmycin biosynthesis seems to involve activation as well as repression. In-frame deletion of a KR6 domain generated the nanchangmycin aglycone with loss of 4-O-methyl-L-rhodinose and antibacterial activity, in agreement with the assignments of the PIKS domains catalyzing specific biosynthetic, steps. [References: 55]
机译：用于聚醚南昌霉素生物合成的PKS基因被组织为编码两组蛋白质（分别为6个和7个ORF），但被编码差向异构酶，环氧化酶和环氧水解酶的独立ORF隔开，尤其是独立的ACP 。 PIKS模块之一缺少相应的ACP。我们提出当聚酮化合物链在释放前仍锚定在独立的ACP上时，发生氧化环化形成聚醚结构的过程。 4-0-甲基-L-若丹糖的生物合成及其转糖基化涉及四个推定的基因，而南昌霉素生物合成的调控似乎涉及激活和抑制。 KR6结构域的框内缺失产生南昌霉素糖苷配基，具有4-O-甲基-L-罗丹糖的损失和抗菌活性，这与催化特定生物合成步骤的PIKS结构域的分配相符。 [参考：55]

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