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首页> 外文期刊>Journal of Aerosol Science >Matrix-assisted laser desorption/ionisation aerosol time-of-flight mass spectrometry for the analysis of bioaerosols:development of a fast detector for airborne biological pathogens
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Matrix-assisted laser desorption/ionisation aerosol time-of-flight mass spectrometry for the analysis of bioaerosols:development of a fast detector for airborne biological pathogens

机译：用于分析生物气溶胶的基质辅助激光解吸/电离气溶胶飞行时间质谱：开发用于机载生物病原体的快速检测器

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摘要

A new aerosol time-of-flight mass spectrometer (ATOFMS) was developed for the near real-time analysis of single bioaerosol particles.The system combines ATOFMS with laser-induced fluorescence selection and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry.The influences of laser power and sample preparation on the quality of the mass spectra have been investigated.A narrow range of laser fluence gives the optimal signals in the mass spectra.On-line application of ferulic acid provides spectra with quality comparable to other preparation methods.The system can analyze aerosolized proteinaceous material up to 20,000 Da,which is the range of the molecular masses of marker ions of bacteria.The results of a preliminary test with spores of B.subtilis var niger show a good prospect for application in the identification of single bacteria and spores.The obtained spectra are promising,although more research is necessary to increase the resolution and accuracy of the spectra.
机译：开发了一种新的气溶胶飞行时间质谱仪（ATOFMS），用于对单个生物气溶胶颗粒进行近实时分析，该系统将ATOFMS与激光诱导的荧光选择和基质辅助激光解吸/电离（MALDI）质谱仪相结合研究了激光功率和样品制备对质谱图质量的影响。狭窄的激光注量范围在质谱图中提供了最佳信号。在线应用阿魏酸可提供质量与其他制备法相当的质谱图该方法可以分析高达20,000 Da的气溶胶化蛋白质物质，这是细菌标记离子的分子量范围。枯草芽孢杆菌孢子的初步测试结果显示出良好的应用前景。鉴定单个细菌和孢子。获得的光谱很有希望，尽管需要进行更多的研究以提高光谱的分辨率和准确性。

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