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首页> 外文期刊>Journal of AOAC International >Determination of isoflavone content in soy, red clover, and kudzu dietary supplement materials by liquid chromatography-particle beam/electron ionization mass spectrometry.
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Determination of isoflavone content in soy, red clover, and kudzu dietary supplement materials by liquid chromatography-particle beam/electron ionization mass spectrometry.

机译：液相色谱-粒子束/电子电离质谱法测定大豆，红三叶草和葛根膳食补充剂中的异黄酮含量。

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摘要

Increased consumption of dietary supplements brings about important requirements of analytical methods to allow accurate and precise measurements of the chemical composition of these botanical materials. Presented here is the isoflavone content in proposed National Institute of Standards and Technology standard reference materials (SRMs) determined by LC-particle beam/electron ionization MS. Botanical materials (soy, red clover, and kudzu) are characterized for the content of a suite of five isoflavones (puerarin, daidzein, genistein, formononetin, and biochanin A). These compounds are of interest due to correlations with certain health benefits. An RP chromatographic separation was first optimized using UV-Vis spectrophotometric detection. The LC output was then introduced to an electron ionization source using a particle beam interface on an Extrel Benchmark MS system. The separation was carried out using a commercial C18 column and a linear gradient using water and methanol (both containing 0.1%) trifluoroacetic acid as mobile phases A and B, at a flow rate of 1.0 mL/min over 40 min. LOD values for the isoflavones were determined to be at the ng level. Quantitation was performed using an internal standard (IS) approach with 7-hydroxy-4-chromone as the IS compound. The levels of isoflavones in the botanical products were determined for the proposed SRMs.
机译：膳食补充剂消耗量的增加带来了对分析方法的重要要求，以允许对这些植物性材料的化学成分进行准确而精确的测量。本文介绍的是拟议中的美国国家标准与技术研究院标准异物（SRM）中的异黄酮含量，其通过LC粒子束/电子电离MS测定。植物材料（大豆，红三叶草和葛根）的特征在于一组五种异黄酮（葛根素，大豆苷，金雀异黄素，formononetin和生物素A）的含量。这些化合物由于与某些健康益处相关而受到关注。首先使用UV-Vis分光光度法对RP色谱分离进行优化。然后使用Extrel Benchmark MS系统上的粒子束界面将LC输出引入电子电离源。使用市售的C 18 色谱柱进行分离，并使用水和甲醇（均含0.1％）三氟乙酸作为流动相A和B进行线性梯度洗脱，流速为1.0 mL / min超过40分钟异黄酮的LOD值确定为ng级。使用内标（IS）方法以7-羟基-4-色酮作为IS化合物进行定量。确定了拟议的SRM中植物产品中异黄酮的含量。

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