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首页> 外文期刊>Journal of Applied Polymer Science >A polyacrylic acid as a carrier for immobilization of penicillin acylase
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A polyacrylic acid as a carrier for immobilization of penicillin acylase

机译：聚丙烯酸作为固定化青霉素酰基转移酶的载体

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摘要

A copolymer of acrylic acid with divinylbenzene was synthesized by suspension polymerization. This polymer is an effective carrier. Penicillin acylase was immobilized on this carrier to convert benzylpenicillin to 6-aminopenicillanic acid, which may be employed in the manufacture of semisynthetic penicillins. Factors that affect the activity of immobilized penicillin acrylase, such as temperature, pH, and amount of native enzyme, were studied. Under suitable conditions, the activity and activity recovery of the immobilized enzyme were 3100 U/g (dry carrier, p-dimethylaminobenzaldehyde method) and 59.7%, respectively. The immobilized penicillin acylase shows a remarkable increase in stability. At 40degreesC and pH 8.0 the value of the kinetic Michaelis-Menten constant (K-m) of the immobilized enzyme is 2.8 x 10(-1) mol/L, and the value of activation energy of enzyme catalysis is 71.5 kJ/mol. (C) 2002 Wiley Periodicals, Inc. [References: 5]
机译：通过悬浮聚合合成丙烯酸与二乙烯基苯的共聚物。该聚合物是有效的载体。将青霉素酰基转移酶固定在该载体上，以将苄青霉素转化为6-氨基青霉酸，其可用于制造半合成青霉素。研究了影响固定化青霉素丙烯酸酶活性的因素，例如温度，pH和天然酶的量。在合适的条件下，固定化酶的活性和活性恢复分别为3100 U / g（干载体，对二甲基氨基苯甲醛法）和59.7％。固定化的青霉素酰基转移酶显示出明显的稳定性增加。在40℃和pH 8.0下，固定化酶的动力学Michaelis-Menten常数（K-m）值为2.8 x 10（-1）mol / L，酶催化的活化能值为71.5 kJ / mol。 （C）2002 Wiley Periodicals，Inc. [参考：5]

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