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首页> 外文期刊>Journal of Archaeological Science >Next generation sequencing of DNA in 3300-year-old charred cereal grains
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Next generation sequencing of DNA in 3300-year-old charred cereal grains

机译：具有3300年历史的炭化谷物中DNA的下一代测序

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摘要

We investigated whether 'next generation' methods can be used to sequence ancient DNA molecules in charred cereal grains. We prepared a DNA extract from a mixed sample of barley, einkorn, emmer and broomcorn millet, taken from a 3300-year-old assemblage of charred cereal grains from Assiros Toumba, Greece. Using the SOLiD 5500 system, we obtained 21,112,844 unique sequence reads. Of these, 178,779 had a 75% or greater nucleotide sequence similarity with one or more entries in the full nucleotide sequence database; 496 of these matches were to previously reported sequences from barley, einkorn, emmer, broomcorn millet or related species such as hexaploid wheat. The unique reads were also compared with a database comprising only wheat sequences. This analysis identified 1658 charred grain sequences that had 90% or greater similarity with segments of the wheat genome. The presence of barley, wheat and millet sequences in the next generation dataset confirms the presence of ancient DNA in this charred grain assemblage. Enrichment of extracts by hybridization capture or equivalent methods is likely to enable sequences to be obtained for entire genes and other genomic regions of interest.
机译：我们研究了“下一代”方法是否可用于对炭化谷物中的古代DNA分子进行测序。我们从大麦，einkorn，emmer和b帚小米的混合样品中提取了DNA提取物，该样品取自3300年前来自希腊阿西罗斯·图姆巴的烧焦谷物。使用SOLiD 5500系统，我们获得了21,112,844个独特的序列读数。其中，178,779与全核苷酸序列数据库中的一个或多个条目具有75％或更高的核苷酸序列相似性;这些匹配中的496个与以前报道的来自大麦，einkorn，emmer，b帚小米或相关物种（如六倍体小麦）的序列有关。还将独特的读数与仅包含小麦序列的数据库进行比较。这项分析确定了1658个与小麦基因组片段具有90％或更高相似度的炭化谷物序列。下一代数据集中存在大麦，小麦和小米序列，证实了这种烧焦谷物组合中存在古代DNA。通过杂交捕获或等效方法富集提取物可能使完整基因和其他目的基因组区域的序列得以获得。

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