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首页> 外文期刊>Journal of applied toxicology >Interaction between silver nanoparticles of 20nm (AgNP20) and human neutrophils: Induction of apoptosis and inhibition of de novo protein synthesis by AgNP20 aggregates
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Interaction between silver nanoparticles of 20nm (AgNP20) and human neutrophils: Induction of apoptosis and inhibition of de novo protein synthesis by AgNP20 aggregates

机译：20nm银纳米颗粒（AgNP20）与人类嗜中性粒细胞之间的相互作用：AgNP20聚集体诱导细胞凋亡并抑制从头合成蛋白质

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摘要

Cytotoxic and proinflammatory properties of silver nanoparticles (AgNPs) have been reported in few studies but the direct interaction between AgNPs and neutrophils, which play a key role in inflammation, has never been documented. Here, we examined the role of AgNPs with a starting size of 20nm (AgNP20) in human neutrophils. Using dynamic light scattering for the characterization of NPs suspended under identical conditions to those used for in vitro experiments, we found that, at 10μgml-1, 92% of AgNP20 possess a diameter of 17.1nm but, at 100μgml-1, a tri-modal size distribution with large aggregates was observed ( 500nm). Neutrophil cell size increased when treated with AgNP20 and transmission electronic microscopy experiments revealed that AgNP20 can rapidly interact with the cell membrane, penetrate neutrophils, localize in vacuole-like structures, and be randomly distributed in the cytosol after 24h. Treatment with 100μgml-1 AgNP20 for 24h (but not 10μgml-1) increased the neutrophil apoptotic rate and inhibited de novo protein synthesis. We conclude that AgNP20 induced apoptosis and can act as potent inhibitors of de novo protein synthesis at 100, but not 10μgml-1 in human neutrophils.
机译：银纳米颗粒（AgNPs）的细胞毒性和促炎特性在少数研究中已有报道，但在炎症中起关键作用的AgNPs和嗜中性粒细胞之间的直接相互作用尚未见文献记载。在这里，我们研究了人类中性粒细胞中起始大小为20nm的AgNPs（AgNP20）的作用。使用动态光散射表征在与体外实验条件相同的条件下悬浮的NP，我们发现，在10μgml-1的条件下，有92％的AgNP20的直径为17.1nm，而在100μgml-1的条件下，三价观察到具有大聚集体的模态尺寸分布（> 500nm）。用AgNP20处理后嗜中性白血球细胞大小增加，透射电子显微镜实验表明，AgNP20可以与细胞膜快速相互作用，穿透嗜中性白细胞，定位于液泡状结构中，并在24h后随机分布在细胞质中。用100μgml-1AgNP20处理24h（而不是10μgml-1）可增加中性粒细胞的凋亡率并抑制从头蛋白质合成。我们得出的结论是，AgNP20诱导细胞凋亡，并且可以在人类嗜中性粒细胞中以100而不是10μgml-1的强度从头合成蛋白质，而并非如此。

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