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首页> 外文期刊>Journal of Applied Ichthyology >The effects of extenders and sperm-egg ratios on fertilizing ability of cryopreserved testicular sperm of northern pike (Esox lucius L.)
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The effects of extenders and sperm-egg ratios on fertilizing ability of cryopreserved testicular sperm of northern pike (Esox lucius L.)

机译：补充剂和精卵比对北派克低温保存的睾丸精子受精能力的影响

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摘要

The effects of four different extenders and two sperm-to-egg ratios on fertilizing ability of cryopreserved testicular sperm of northern pike (Esox lucius L.) were tested in the present study. Testicular sperm was diluted with each of the four extenders (0.6 M sucrose + 15% DMSO, 0.3 M glucose + 15% DMSO, 0.6 M sucrose + 10% methanol and 0.3 M glucose + 10% methanol, all supplemented with 10% egg yolk and 1.7 g KCl L-1) and frozen in 0.5-ml straws at 2 cm above the surface of liquid nitrogen and thawed at 25 degrees C for 30 s. Then 125 mu l or 50 mu l of frozen-thawed testicular sperm was poured onto about 1250 eggs for fertilization. The results showed that both sperm-to-egg ratio and diluent had no significant influence on cryopreservation efficiency of testicular sperm, whereas cryoprotectant had a significant influence. The highest fertilization rate (92.2%) was obtained from testicular sperm cryopreserved in glucose-based extender containing 10% methanol at a sperm-to-egg ratio of 1 x 10(6) spermatozoa per egg. The results indicated that glucose-based extender containing 10% methanol, 10% egg yolk and 1.7 g KCl L-1 was a useful combination.
机译：在本研究中，测试了四种不同的补充剂和两种精子/卵子比率对冷冻的北派克（Esox lucius L.）睾丸精子的受精能力的影响。用四种增量剂（0.6 M蔗糖+ 15％DMSO，0.3 M葡萄糖+ 15％DMSO，0.6 M蔗糖+ 10％甲醇和0.3 M葡萄糖+ 10％甲醇，均添加10％蛋黄）稀释睾丸精子。和1.7 g KCl L-1），在液氮表面上方2 cm的0.5 ml吸管中冷冻，并在25摄氏度下融化30 s。然后将125μl或50μl冻融的睾丸精子倒在约1250个卵上进行受精。结果表明，精卵比和稀释剂对睾丸精子的冷冻保存效率没有显着影响，而冷冻保护剂则有显着影响。受精率最高的是睾丸精子，其冷冻保存在含10％甲醇的葡萄糖基填充剂中，精子与蛋的比例为每个鸡蛋1 x 10（6）精子。结果表明，含有10％甲醇，10％蛋黄和1.7 g KCl L-1的葡萄糖基增量剂是有用的组合。

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