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首页> 外文期刊>Journal of Applied Ichthyology >Sperm motility parameters for Steindachneridion parahybae based on open-source software
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Sperm motility parameters for Steindachneridion parahybae based on open-source software

机译：基于开源软件的副鞭毛虫精子活力参数

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摘要

The objective of this work was to evaluate the sperm motility of 13 Steindachneridion parahybae males using open-source software (ImageJ/CASA plugin). The sperm activation procedure and image capture were initiated after semen collection. Four experimental phases were defined from the videos captured of each male as follows: (i) standardization of a dialogue box generated by the CASA plugin within ImageJ; (ii) frame numbers used to perform the analysis; (iii) post-activation motility between 10 and 20 s with analysis at each 1 s; and (iv) post-activation motility between 10 and 50 s with analysis at each 10 s. The settings used in the CASA dialogue box were satisfactory, and the results were consistent. These analyses should be performed using 50 frames immediately after sperm activation because spermatozoa quickly lose their vigor. At 10 s post-activation, 89.1% motile sperm was observed with 107.2 mu m s(-1) curvilinear velocity, 83.6 mu m s(-1) average path velocity, 77.1 mu m s(-1) straight line velocity; 91.6% were of straightness and 77.1% of wobble. The CASA plugin within ImageJ can be applied in sperm analysis of the study species by using the established settings.
机译：这项工作的目的是使用开源软件（ImageJ / CASA插件）评估13名Steindachneridion parahybae雄性的精子运动能力。精液收集后开始精子激活程序和图像捕获。从捕获的每个男性的视频中定义了四个实验阶段，如下所示：（i）由ImageJ中的CASA插件生成的对话框的标准化; （ii）用于执行分析的帧号; （iii）激活后的动力在10到20 s之间，每1 s进行一次分析; （iv）激活后的运动能力在10到50 s之间，每10 s进行一次分析。 CASA对话框中使用的设置令人满意，并且结果一致。这些分析应在精子激活后立即使用50帧进行，因为精子会迅速失去活力。在激活后10 s，观察到89.1％的活动精子，曲线速度为107.2μm s（-1），平均路径速度为83.6μm s（-1），直线速度为77.1μm s（-1）。平直度为91.6％，摆动度为77.1％。通过使用已建立的设置，ImageJ中的CASA插件可用于研究物种的精子分析。

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