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首页> 外文期刊>Journal of biochemical and molecular toxicology >RNA-Seq Reveals Acute Manganese Exposure Increases Endoplasmic Reticulum Related and Lipocalin mRNAs in Caenorhabditis elegans
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RNA-Seq Reveals Acute Manganese Exposure Increases Endoplasmic Reticulum Related and Lipocalin mRNAs in Caenorhabditis elegans

机译：RNA-Seq显示急性锰暴露增加秀丽隐杆线虫的内质网相关和脂质蛋白mRNA。

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Manganese (Mn) is an essential nutrient; nonetheless, excessive amounts can accumulate in brain tissues causing manganism, a severe neurological condition. Previous studies have suggested oxidative stress, mitochondria dysfunction, and impaired metabolism pathways as routes for Mn toxicity. Here, we used the nematode Caenorhabditis elegans to analyze gene expression changes after acute Mn exposure using RNA-Seq. L1 stage animals were exposed to 50 mM MnCl2 for 30 min and analyzed at L4. We identified 746 up- and 1828 downregulated genes (FDR corrected p < 0.05; two-fold change) that included endoplasmic reticulum related abu and fkb family genes, as well as six of seven lipocalin-related (lpr) family members. These were also verified by qRT-PCR. RNA interference of lpr-5 showed a dramatic increase in whole body vulnerability to Mn exposure. Our studies demonstrate that Mn exposure alters gene transcriptional levels in different cell stress pathways that may ultimately contribute to its toxic effects.
机译：锰（Mn）是必不可少的营养素。但是，过量的蛋白质会积聚在脑组织中，引起锰症，这是一种严重的神经系统疾病。先前的研究表明，氧化应激，线粒体功能障碍和代谢途径受损是锰毒性的途径。在这里，我们使用线虫秀丽隐杆线虫（Caenorhabditis elegans）分析了使用RNA-Seq暴露于急性锰后的基因表达变化。将L1期动物暴露于50 mM MnCl2中30分钟，并在L4处进行分析。我们鉴定了746个上调基因和1828个下调基因（FDR校正p <0.05;两倍变化），其中包括内质网相关的abu和fkb家族基因，以及七个脂蛋白相关（lpr）家族成员中的六个。这些也通过qRT-PCR验证。 lpr-5的RNA干扰显示出全身对锰暴露的脆弱性显着增加。我们的研究表明，Mn暴露会改变不同细胞应激途径中的基因转录水平，这可能最终导致其毒性作用。

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