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首页> 外文期刊>Journal of biomaterials and tissue engineering >Calcium Sulfate Hemihydrate/Mineralized Collagen for Bone Tissue Engineering: In Vitro Release and In Vivo Bone Regeneration Studies
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Calcium Sulfate Hemihydrate/Mineralized Collagen for Bone Tissue Engineering: In Vitro Release and In Vivo Bone Regeneration Studies

机译：硫酸钙半水合物/矿化胶原蛋白用于骨组织工程：体外释放和体内骨再生研究

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The objective of this study was to investigate the feasibility of calcium sulfate hemihydrate/mineralized collagen (CSHHAC) as a carrier for sustained release of BMP-2 derived peptide and in vivo bone formation capacity. A peptide was fabricated according to sequence of peptide in BMP-2 active core region by the FMOC/tBu solid-phase peptide synthesis. CSHHAC was prepared and observed with scanning electron microscope. BMP-2 derived peptide was introduced into CSHHAC. For observing the release kinetics of BMP-2 derived peptide, the released content from CSHHAC was detected using high performance liquid chromatography at different time points. CSHHAC loaded with BMP-2 derived peptide as Group A and CSHHAC without BMP-2 derived peptide as Group B were respectively implanted into a critical size defect model in the femoral condyle of rabbit. At the same time, we set up the control group as group C. Up to 4th, 8th and 12th week after implantation, the rabbits were killed in batch, and the samples were harvested and detected by X-rays, Micro CT, and histological observation. The released character of BMP-2 derived peptide was that an initial burst release (32.9%) was observed in the first day, followed by a sustained release and reached 100% by day 25. The results of X-rays, Micro CT and histological observation indicated that more new bone was formed in Group A, and the difference was marked at each stage. Through histological quantitative analysis, a significant difference in the new bone formation was found in these three groups. Consequently, we conclude that CSHHAC can be served as a carrier for sustained release of BMP-2 derived peptide and CSHHAC loaded with BMP-2 derived peptide has a great potential in bone tissue engineering.
机译：这项研究的目的是研究半水硫酸钙/矿化胶原蛋白（CSH / nHAC）作为载体持续释放BMP-2衍生肽和体内骨骼形成能力的可行性。通过FMOC / tBu固相肽合成，根据BMP-2活性核心区中肽的序列制备肽。制备了CSH / nHAC，并用扫描电子显微镜观察。将BMP-2衍生肽引入CSH / nHAC。为了观察BMP-2衍生肽的释放动力学，使用高效液相色谱在不同时间点检测了CSH / nHAC的释放量。分别将载有BMP-2衍生肽的CSH / nHAC（A组）和不含BMP-2衍生肽的CSH / nHAC（B组）植入兔股骨a的临界尺寸缺损模型中。同时，将对照组设为C组。植入后第4、8、12周，分批处死兔子，取X线片，Micro CT及组织学检查标本。观察。 BMP-2衍生肽的释放特征是在第一天观察到最初的突释释放（32.9％），然后持续释放，到第25天达到100％。X射线，Micro CT和组织学结果观察表明，A组中形成了更多的新骨，并且在每个阶段都有明显的差异。通过组织学定量分析，在这三组中发现了新骨形成的显着差异。因此，我们得出结论，CSH / nHAC可以作为BMP-2衍生肽的持续释放的载体，而载有BMP-2衍生肽的CSH / nHAC在骨组织工程中具有巨大的潜力。

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