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首页> 外文期刊>Journal of Biotechnology >Development of tailor-made glycidyl methacrylate-divinyl benzene copolymerfor immobilization of D-amino acid oxidase from Aspergillus species strain020 and its application in the bioconversion of cephalosporin C
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Development of tailor-made glycidyl methacrylate-divinyl benzene copolymerfor immobilization of D-amino acid oxidase from Aspergillus species strain020 and its application in the bioconversion of cephalosporin C

机译：定制的固定化曲霉菌种D-氨基酸氧化酶的甲基丙烯酸缩水甘油酯-二乙烯基苯共聚物的研制及其在头孢菌素C生物转化中的应用

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摘要

A tailor-made glycidyl methacrylate-divinyl benzene (GMA-DVB) copolymer PC-3 was evolved by studying the effect of synthesis variables on binding and expression of D-amino acid oxidase (DAAO) from Aspergillus species strain 020. Almost quantitative binding (100%) and a high yield of immobilization per unit of enzyme loaded was achieved. Optimum pH, optimum temperature and K-m for soluble and IME-DAAO preparations were 8.0-8.5 and 8.0; 30 and 37 degrees C; and 0.73 and 2.78 mM for ceph C, respectively. The developed immobilized DAAO was used for the production of glutaryl-7-aminocephalosporanic acid from cephalosporin C (ceph C). A high conversion of > 95% was achieved by using 3% (w/v) solution of ceph C, 48 U of DAAO per g of ceph C, keeping dissolved oxygen level above 50%, maintaining the pH between 7.6 and 7.8 and temperature at 24 degrees C. The immobilized DAAO was used for 60 cycles in a stirred tank reactor.
机译：通过研究合成变量对曲霉菌属菌株020的D-氨基酸氧化酶（DAAO）结合和表达的影响，开发了量身定制的甲基丙烯酸缩水甘油酯-二乙烯基苯（GMA-DVB）共聚物PC-3。 100％）和每单位负载酶的固定化率高。可溶性和IME-DAAO制剂的最佳pH，最佳温度和K-m为8.0-8.5和8.0; 30和37摄氏度; ceph C分别为0.73和2.78 mM。发达的固定化DAAO用于从头孢菌素C（ceph C）生产戊二酰7-氨基头孢烷酸。通过使用3％（w / v）的头孢C溶液，每克头孢C含48 U DAAO，保持溶解氧水平在50％以上，pH值保持在7.6至7.8和温度不变，可以实现> 95％的高转化率在24℃下将固定的DAAO在搅拌釜反应器中使用60个循环。

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