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首页> 外文期刊>Journal of Biotechnology >Transcriptional analysis of catabolite repression in Clostridium acetobutylicum growing on mixtures of D-glucose and D-xylose
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Transcriptional analysis of catabolite repression in Clostridium acetobutylicum growing on mixtures of D-glucose and D-xylose

机译：D-葡萄糖和D-木糖混合物上生长的丙酮丁醇梭菌中分解代谢物阻遏的转录分析

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Clostridium acetobutylicum is a strict anaerobic organism that is used for biotechnological butanol fermentation. It ferments various hexoses and pentoses to solvents but prefers glucose presumably using a catabolite repression mechanism. Accordingly during growth on a mixture of D-glucose and D-xylose a typical diauxic growth pattern was observed. We used DNA microarrays and real-time RT-PCR to study gene expression during growth on D-glucose, D-xylose mixtures on a defined minimal medium together with monitoring substrate consumption and product formation. We identified two putative operons involved in D-xylose degradation. The first operon (CAC1344-CAC1349) includes a transporter, a xylulose-kinase, a transaldolase, a transketolase, an aldose-1-epimerase and a putative xylose isomerase that has been annotated as an arabinose isomerase. This operon is induced by D-xylose but was catabolite repressed by D-glucose. A second operon (CAC2610-CAC2612) consists of a xylulose-kinase. a hypothetical protein and a gene that has been annotated as a L-fucose isomerase that might in fact code for a xylose isomerase. This operon was induced by D-xylose but was not subject to catabolite repression. In accordance with these results we identified a CRE site in the catabolite repressed operon but not in the operon that was not subject to catabolite repression
机译：丙酮丁醇梭菌是严格的厌氧生物，用于生物技术丁醇发酵。它可以将各种己糖和戊糖发酵成溶剂，但大概是使用分解代谢物抑制机制来偏爱葡萄糖。因此，在D-葡萄糖和D-木糖的混合物上生长期间，观察到典型的双峰生长模式。我们使用DNA微阵列和实时RT-PCR研究了在限定的基本培养基上的D-葡萄糖，D-木糖混合物上生长期间的基因表达，并监测了底物的消耗和产物的形成。我们确定了两个假定的操纵子参与D-木糖降解。第一个操纵子（CAC1344-CAC1349）包括转运蛋白，木酮糖激酶，反式醛缩酶，反式酮醇酶，醛糖-1-表异构酶和假定的木糖异构酶（已标注为阿拉伯糖异构酶）。该操纵子由D-木糖诱导，但被D-葡萄糖抑制。第二个操纵子（CAC2610-CAC2612）由木酮糖激酶组成。一种假设的蛋白质和一个已被注释为L-岩藻糖异构酶的基因，实际上可能编码木糖异构酶。该操纵子由D-木糖诱导，但不受分解代谢物的抑制。根据这些结果，我们在受分解代谢物阻抑的操纵子中确定了一个CRE位点，但未在不受分解代谢物阻抑的操纵子中确定了一个CRE位点

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