• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Biotechnology >Expanded bed protein A affinity chromatography of a recombinant humanizedmonoclonal antibody: process development, operation, and comparison with apacked bed method
【24h】

Expanded bed protein A affinity chromatography of a recombinant humanizedmonoclonal antibody: process development, operation, and comparison with apacked bed method

机译：重组人源化单克隆抗体的扩展床蛋白A亲和色谱：工艺开发，操作以及与多床方法的比较

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We show that expanded bed protein A affinity chromatography using Streamline rProtein A. media is an efficient method for purifying a recombinant humanized monoclonal antibody from unclarified Chinese hamster ovary cell culture fluid and that it provides purification performance comparable to using a packed bed. We determined that the dynamic capacity of the expanded bed media is related to flow rate (measured in column volumes per hour) by a power function, which allows a high capacity at a low flow rate. At 250 cm h(-1) with a 25 cm bed height (10 column volumes h(-1)), the dynamic capacity is 30 g l(-1). The yield and purity (measured by the amount of host cell proteins, DNA, SDS-PAGE, and turbidity) of the antibody purified by expanded bed is comparable to the yield and purity obtained on a standard packed bed method using Prosep A media.
机译：我们显示，使用Streamline rProtein A.培养基的扩展床蛋白A亲和色谱法是一种从未澄清的中国仓鼠卵巢细胞培养液中纯化重组人源化单克隆抗体的有效方法，并且其纯化性能可与使用填充床媲美。我们通过功率函数确定了膨胀床介质的动态容量与流速（以每小时每小时柱体积衡量）相关，从而可以在低流速下实现高容量。在250 cm h（-1）和25 cm床高（10个柱体积h（-1））下，动态容量为30 g l（-1）。通过膨胀床纯化的抗体的产量和纯度（通过宿主细胞蛋白质，DNA，SDS-PAGE和浊度的量来衡量）与使用Prosep A培养基在标准填充床方法中获得的产量和纯度相当。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号