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首页> 外文期刊>Journal of Biomechanics >Prediction of the micro-fluid dynamic environment imposed to three-dimensional engineered cell systems in bioreactors.
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Prediction of the micro-fluid dynamic environment imposed to three-dimensional engineered cell systems in bioreactors.

机译：对施加于生物反应器中三维工程细胞系统的微流体动态环境的预测。

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Bioreactors allowing culture medium perfusion overcome diffusion limitations associated with static culturing and provide flow-mediated mechanical stimuli. The hydrodynamic stress imposed to cells will depend not only on the culture medium flow rate, but also on the scaffold three-dimensional (3D) micro-architecture. We developed a CFD model of the flow of culture medium through a 3D scaffold of homogeneous geometry, with the aim of predicting the shear stress acting on cells as a function of parameters that can be controlled during the scaffold fabrication process, such as the scaffold porosity and the pore size, and during the cell culture, such as the medium flow rate and the diameter of the perfused scaffold section. We built three groups of models corresponding to three pore sizes: 50, 100 and 150 microm. Each group was made of four models corresponding to 59%, 65%, 77%, and 89% porosity. A commercial finite-element code was used to set up and solve the problem and to analyze the results. The modevalue of shear stress varied between 2 and 5 mPa, and was obtained for a circular scaffold of 15.5 mm diameter, perfused by a flow rate of 0.5 ml/min. The simulations showed that the pore size is a variable strongly influencing the predicted shear stress level, whereas the porosity is a variable strongly affecting the statistical distribution of the shear stresses, but not their magnitude. Our results provide a basis for the completion of more exhaustive quantitative studies to further assess the relationship between perfusion, at known micro-fluid dynamic conditions, and tissue growth in vitro.
机译：允许培养基灌注的生物反应器克服了与静态培养相关的扩散限制，并提供了流介导的机械刺激。施加到细胞上的流体动力应力不仅取决于培养基的流速，还取决于支架的三维（3D）微结构。我们开发了通过均质几何形状的3D支架的培养基流的CFD模型，目的是预测作用在细胞上的剪切应力与在支架制造过程中可以控制的参数（例如支架孔隙率）的函数关系孔的大小以及细胞培养过程中的流速，例如灌注支架的培养基流速和直径。我们建立了三组模型，分别对应三种孔径：50、100和150微米。每组由四个模型组成，分别对应于59％，65％，77％和89％的孔隙度。使用商业有限元代码来设置和解决该问题并分析结果。剪切应力的众数值在2至5 mPa之间变化，这是通过以0.5 ml / min的流速灌注的直径为15.5 mm的圆形支架获得的。模拟表明，孔径是一个变量，强烈影响预测的剪应力水平，而孔隙度是一个变量，强烈影响剪应力的统计分布，但不影响其大小。我们的结果为完成更详尽的定量研究提供了基础，以进一步评估已知微流体动态条件下的灌注与体外组织生长之间的关系。

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