Oxidized low-density lipoprotein (Ox-LDL) impacts on erythrocyte viscoelasticity and its molecular mechanism.

Wang X; Yang L; Liu Y

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Oxidized low-density lipoprotein (Ox-LDL) impacts on erythrocyte viscoelasticity and its molecular mechanism.

机译：氧化的低密度脂蛋白（Ox-LDL）对红细胞粘弹性及其分子机制的影响。

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The oxidized low-density lipoprotein (Ox-LDL) plays an important role in atherosclerosis, yet it remains unclear if it damages circulating erythrocytes. In this study, erythrocyte deformability and its membrane proteins after Ox-LDL incubations are investigated by micropipette aspiration, thiol radical measurement, and sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Results show that Ox-LDL incubation reduces the erythrocyte deformability, decreases free thiol radical contents in erythrocytes, and induces the cross-linking among membrane proteins. SDS-PAGE analysis reveals a high molecular weight (HMW) complex as well as new bands between spectrins and band 3 and reduced ratios between band 3 and other major membrane skeletal proteins. Analyses indicate that Ox-LDL makes erythrocytes harder to deform through a molecular mechanism by which the oxidation of free thiol radicals forms disulfide bonds among membrane skeletal proteins.

机译：氧化的低密度脂蛋白（Ox-LDL）在动脉粥样硬化中起着重要作用，但尚不清楚它是否损害循环中的红细胞。在这项研究中，通过微量移液器抽吸，硫醇自由基测量和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）研究了Ox-LDL孵育后的红细胞变形能力及其膜蛋白。结果表明，Ox-LDL孵育降低了红细胞的可变形性，降低了红细胞中的游离巯基含量，并诱导了膜蛋白之间的交联。 SDS-PAGE分析揭示了高分子量（HMW）复合物，以及在光谱与条带3之间的新条带以及在条带3与其他主要膜骨骼蛋白之间的比率降低。分析表明，Ox-LDL通过分子机制使红细胞更难变形，通过这种分子机制，游离硫醇基的氧化在膜骨架蛋白之间形成了二硫键。

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《Journal of Biomechanics》 |2009年第14期|共6页
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Wang X; Yang L; Liu Y;
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正文语种 eng
中图分类医用一般科学;生物力学;
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1. Oxidized low-density lipoprotein (Ox-LDL) impacts on erythrocyte viscoelasticity and its molecular mechanism. [J] . Wang X, Yang L, Liu Y Journal of Biomechanics . 2009,第14期

机译：氧化的低密度脂蛋白（Ox-LDL）对红细胞粘弹性及其分子机制的影响。
2. Oxidized low-density lipoprotein (ox-LDL) binding to lectin-like ox-LDL receptor-1 (LOX-1) in cultured bovine articular chondrocytes increases production of intracellular reactive oxygen species (ROS) resulting in the activation of NF-kappaB. [J] . Nishimura S, Akagi M, Yoshida K, Osteoarthritis and cartilage . 2004,第7期

机译：氧化的低密度脂蛋白（ox-LDL）与培养的牛关节软骨细胞中凝集素样ox-LDL受体-1（LOX-1）的结合增加了细胞内活性氧（ROS）的产生，导致NF-kappaB的激活。
3. Upregulation of miR-223 abrogates NLRP3 inflammasome-mediated pyroptosis to attenuate oxidized low-density lipoprotein (ox-LDL)-induced cell death in human vascular endothelial cells (ECs) [J] . Wang Xumin, Li Xinwei, Wu Yuhong, In Vitro Cellular and Developmental Biology. Animal: Journal of the Tissues Culture Association . 2020,第8期

机译：MiR-223的上调废除NLRP3炎症般介导的γ唑唑抑制，以衰减氧化低密度脂蛋白（OX-LDL）诱导的人血管内皮细胞（ECS）中的细胞死亡
4. Formation of A^-(hexanoyl)ethanolamine, a novel phosphatidylethanolamine adduct, during the oxidation of erythrocyte membrane and low-density lipoprotein [C] . Kentaro Tsuji, Yoshichika Kawai, Yoji Kato, International Conference on Food Factors . 2004

机译：在红细胞膜和低密度脂蛋白的氧化期间形成^ - （己酰基）乙醇胺，新型磷脂酰乙醇胺加合物
5. Studies of CD36 interacting with fatty acids, oxidized low-density lipoprotein, and the cellular plasma membrane. [D] . Jay, Anthony George. 2017

机译：CD36与脂肪酸，氧化的低密度脂蛋白和细胞质膜相互作用的研究。
6. Astragaloside IV Protects Against Oxidized Low-Density Lipoprotein (ox-LDL)-Induced Endothelial Cell Injury by Reducing Oxidative Stress and Inflammation [O] . Zhongsheng Zhu, Jinyu Li, Xiaorong Zhang 2019

机译：黄芪甲苷IV通过减少氧化应激和炎症反应来保护抗氧化的低密度脂蛋白（ox-LDL）诱导的内皮细胞损伤
7. Oxidized low-density lipoprotein (ox-LDL) binding to lectin-like ox-LDL receptor-1 (LOX-1) in cultured bovine articular chondrocytes increases production of intracellular reactive oxygen species (ROS) resulting in the activation of NF-κB [O] . Nishimura Shunji, Akagi Masao, Yoshida Koji, 2004

机译：氧化的低密度脂蛋白（ox-LDL）与培养的牛关节软骨细胞中凝集素样ox-LDL受体-1（LOX-1）的结合增加了细胞内活性氧（ROS）的产生，导致NF-κB的激活

Oxidized low-density lipoprotein (Ox-LDL) impacts on erythrocyte viscoelasticity and its molecular mechanism.

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