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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Bioactive glass serves as a substrate for maintenance of phenotype of nucleus pulposus cells of the intervertebral disc
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Bioactive glass serves as a substrate for maintenance of phenotype of nucleus pulposus cells of the intervertebral disc

机译：生物活性玻璃作为维持椎间盘髓核细胞表型的基质

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摘要

The objective of the study was to investigate the capability of bioactive glass 45S5 to serve as a substrate for nucleus pulposus cells in vitro. Nucleus pulposus cells were isolated from adult rabbit discs and seeded onto bioactive glass. At selected time intervals, the cells and glass were evaluated. We found that the cells rapidly attached to the substrate, colonizing it within 12 h. By 21 days, they had formed a lawn of cells over the glass substrate. DNA measurements showed a progressive increase in cell number with time. The phenotype was maintained;;the cells expressed aggrecan, and collagen type II and I, but were negative for collagen type X. CD44, a cell-surface glycoprotein that binds hyaluronate, was also expressed by these cells. Electron dispersive X-ray analysis and Fourier transform infrared spectroscopy revealed calcium phosphate-rich layer formation on the substrate surface. The results of this study suggest that nucleus pulposus cell proliferation may be an anchorage dependent event, and that the cells use the calcium phosphate-rich layer to facilitate cell adhesion, and subsequent proliferation. These findings point to the importance of the conditioned bioactive glass as a substrate for nucleus pulposus cells.
机译：该研究的目的是研究生物活性玻璃45S5用作体外髓核细胞底物的能力。从成年兔椎间盘分离髓核细胞，并接种到生物活性玻璃上。在选定的时间间隔，评估电池和玻璃。我们发现细胞迅速附着在基质上，在12小时内定植。到21天时，他们已经在玻璃基板上形成了小单元的草坪。 DNA测量显示细胞数随时间逐渐增加。该表型得以维持;细胞表达聚集蛋白聚糖，II型和I型胶原蛋白，但对X型胶原蛋白呈阴性。这些细胞还表达结合透明质酸盐的细胞表面糖蛋白CD44。电子弥散X射线分析和傅立叶变换红外光谱显示在基材表面上形成了磷酸钙富集层。这项研究的结果表明髓核细胞增殖可能是依赖锚固的事件，并且细胞使用富含磷酸钙的层来促进细胞粘附和随后的增殖。这些发现表明条件生物活性玻璃作为髓核细胞底物的重要性。

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